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Procaine-induced maturation of Xenopus oocytes is mediated by a transient activation of M-Phase promoting factor

Published online by Cambridge University Press:  26 September 2008

Stéphane Flament ,
Jean-François Bodart ,
Edith Browaeys ,
Marc Bertout ,
Arlette Rousseau ,
Julian Gannon  and
Jean-Pierre Vilain
Stéphane Flament*
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
Jean-François Bodart
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
Edith Browaeys
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
Marc Bertout
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
Arlette Rousseau
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
Julian Gannon
Affiliation:
Imperial Cancer Research Fund Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
Jean-Pierre Vilain
Affiliation:
Centre de Biologie Cellulaire, Université de Lille 1, Villeneuve d'Ascq, France.
*
Stéphane Flament, Centre de Biologie Cellulaire, Unité de Dynamique des cellules embryonnaires et cancéreuses, Laboratoire de Biologie du Développement, EA DRED 1033, Université de Lille 1, SN3, F-59655 Villeneuve d'Ascq cedex, France. Telephone: +33 20-43-68-67. Fax: +33 20-43-40-38. e-mail: [email protected].
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Summary

We have recently shown that the incubation of Xenopus laevis oocytes in procaine-containing solutions induced germinal vesicle breakdown without white spot formation and, in some cases, with the appearance of spindle and chromosomes in the cytoplasm. The present study was performed to determine whether M-phase promoting factor was involved in this unusual maturation. Procaine failed to induce maturation in the presence of 6-dimethylamino purine or roscovitine, which are both known to inhibit p34cdc2kinase. Histone H1 kinase activity was detected in procaine-treated oocytes but it was always lower than in progesterone-treated controls. A shift in p34cdc2 was observed in oocytes that had been exposed to procaine for 16h, but it was not detected in those exposed for 24h. Finally, cytoplasm transfer experiments demonstrated that the maturation promoting activity that occurred in oocytes incubated in procaine for 16h could induce maturation of recipient stage VI oocytes. This transferable activity was weaker than that from progesterone-treated controls since only 30% of the recipients underwent germinal vesicle breakdown and only a few spindles were observed, which were not always correctly located. Taken together these results demonstrate that M-phase promoting factor is involved in the procaine maturing effect despite some differences compared with progesterone-treated oocytes which might explain the particular type of maturation induced by this substance. The discovery of the mechanisms by which procaine is able to activate M-phase promoting factor might now help in the understanding of some steps in progesterone-induced maturation that have still to be elucidated.

Keywords

Intracellular pHMaturationMPFProcaineXenopus oocyte
Type
Article
Information
Zygote , Volume 5 , Issue 1 , February 1997 , pp. 11 - 19
Copyright
Copyright © Cambridge University Press 1997

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