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The effects of methyl-β-cyclodextrin on in vitro fertilization and the subsequent development of bovine oocytes

Published online by Cambridge University Press:  24 March 2010

Yoshikazu Nagao ,
Yuki Ohta ,
Hidemi Murakami  and
Yoku Kato
Yoshikazu Nagao*
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Yuki Ohta
Affiliation:
University Farm, Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan. Science Service Inc., Japan.
Hidemi Murakami
Affiliation:
University Farm, Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan.
Yoku Kato
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
*
All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. Tel: +81 285 84 1321. Fax: +81 285 84 1321. e-mail: [email protected]
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Summary

In this study, we investigated the effects of methyl-β-cyclodextrin (MBCD) on in vitro fertilization and the subsequent development of bovine oocytes. Bovine oocytes matured in serum-free medium were inseminated with frozen–thawed sperm pre-incubated in protein-free modified Brackett and Oliphant medium (BO) containing various concentrations of MBCD for various periods. MBCD decreased the frequency of live sperm, however enhanced the capacitation and acrosome reaction of the live sperm. Pre-incubation of sperm with 0.5, 1.0 and 1.5 mM MBCD for 2 and 4 h increased the frequency of normal fertilization. Embryos derived from oocytes fertilized with spermatozoa pre-incubated with MBCD developed normally to the blastocyst stage and term. There were individual differences and similar tendencies in four different sires in terms of the effects of MBCD upon fertilization. These results indicate that the pre-incubation of bovine sperm with MBCD affects viability and capacitation status of the sperm and promotes fertilization in vitro. Embryos derived from oocytes fertilized with sperm pre-incubated with MBCD developed normally to the blastocyst stage and term.

Keywords

BovineDevelopmentFertilizationMethyl-β-cyclodextrinOocyte
Type
Research Article
Information
Zygote , Volume 18 , Issue 4 , November 2010 , pp. 323 - 330
Copyright
Copyright © Cambridge University Press 2010

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