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Effects of jacalin and follicle-stimulating hormone on in vitro goat primordial follicle activation, survival and gene expression

Published online by Cambridge University Press:  29 May 2014

Regislane P. Ribeiro ,
Antonia M.L.R. Portela ,
Anderson W.B. Silva ,
José J.N. Costa ,
José R.S. Passos ,
Ellen V. Cunha ,
Glaucinete B. Souza ,
Márcia V.A. Saraiva ,
Mariana A. M. Donato  and
Christina. A. Peixoto
...Show all authors
Regislane P. Ribeiro
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Antonia M.L.R. Portela
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Anderson W.B. Silva
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
José J.N. Costa
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
José R.S. Passos
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Ellen V. Cunha
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Glaucinete B. Souza
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Márcia V.A. Saraiva
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, CEP 62042–280, Sobral, CE, Brazil.
Mariana A. M. Donato
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
Christina. A. Peixoto
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
Robert van den Hurk
Affiliation:
Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.163, Utrecht, The Netherlands.
José R.V. Silva*
Affiliation:
Biotechnology Nucleus of Sobral – NUBIS, Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041–040, Sobral, CE, Brazil.
*
All correspondence to: J.R.V. Silva. Biotechnology Nucleus of Sobral – NUBIS, Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041–040, Sobral, CE, Brazil. Tel:/Fax: +55 88 36118000. e-mail: [email protected]
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Summary

This study aims to investigate the effects of jacalin and follicle-stimulating hormone (FSH) on activation and survival of goat primordial follicles, as well as on gene expression in cultured ovarian tissue. Ovarian fragments were cultured for 6 days in minimum essential medium (MEM) supplemented with jacalin (10, 25, 50 or 100 μg/ml – Experiment 1) or in MEM supplemented with jacalin (50 μg/ml), FSH (50 ng/ml) or both (Experiment 2). Non-cultured and cultured tissues were processed for histological and ultrastructural analysis. Cultured tissues from Experiment 2 were also stored to evaluate the expression of BMP-15, KL (Kit ligand), c-kit, GDF-9 and proliferating cell nuclear antigen (PCNA) by real-time polymerase chain reaction (PCR). The results of Experiment 1 showed that, compared with tissue that was cultured in control medium, the presence of 50 μg/ml of jacalin increased both the percentages of developing follicles and viability. In Experiment 2, after 6 days, higher percentages of normal follicles were observed in tissue cultured in presence of FSH, jacalin or both, but no synergistic interaction between FSH and jacalin was observed. These substances had no significant effect on the levels of mRNA for BMP-15 and KL, but FSH increased significantly the levels of mRNA for PCNA and c-kit. On the other hand, jacalin reduced the levels of mRNA for GDF-9. In conclusion, jacalin and FSH are able to improve primordial follicle activation and survival after 6 days of culture. Furthermore, presence of FSH increases the expression of mRNA for PCNA and c-kit, but jacalin resulted in lower GDF-9 mRNA expression.

Keywords

CaprineCultureJacalinmRNAOvarian fragmentsPre-antral follicles
Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 537 - 549
Copyright
Copyright © Cambridge University Press 2014 

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