Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-03T00:33:37.607Z Has data issue: false hasContentIssue false
  • English
  • Français

Artificial oocyte activation and human failed-matured oocyte vitrification followed by in vitro maturation

Published online by Cambridge University Press:  26 August 2011

Y. Liu ,
Y.X. Cao ,
Z.G. Zhang  and
Q. Xing
Y. Liu
Affiliation:
Department of Obstetrics and Gynecology, Maternal and Child Care Hospital of Hefei City, Hefei, China.
Y.X. Cao*
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
Z.G. Zhang
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
Q. Xing
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
*
All correspondence to: Yunxia Cao. Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China. Tel: +86 551 2922071. Fax: +86 551 2922071. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The investigation presented in this paper was conducted on the effect of oocytes activation on frozen–thawed human immature oocytes followed by in vitro maturation (IVM). A total of 386 failed-matured oocytes (germinal vesicle (GV) and metaphase I (MI) stages) was randomly divided into two groups: fresh group and vitrification group, GV group and MI group, respectively). The matured oocytes were subject to intracytoplasmic sperm injection (ICSI) after IVM had been carried out. The vitrification group was randomly divided into two groups: controlled and artificial oocyte activation (AOA). The injected oocytes in the controlled group were cultured in cleavage medium. The AOA group oocytes were activated by exposing them to 7% anhydrous alcohol for 6 min then cultured in cleavage medium as well. The rates of fertilization and early embryonic development were compared between the controlled and AOA groups. In MI vitrification group, the high-quality embryo formation rate and blastocyst formation rate were significantly higher in the AOA group than in the controlled group (P < 0.01). In the GV vitrification group, the high-quality embryo formation rate was significantly higher in the AOA group than in the controlled group (P < 0.05). These results indicate that AOA may be good for early embryonic development of vitrified immature human oocytes.

Keywords

DevelopmentFertilizationHuman oocyteOocyte activationVitrification
Type
Research Article
Information
Zygote , Volume 21 , Issue 1 , February 2013 , pp. 71 - 76
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ben-Yosef, D. & Shalgi, R. (1998). Early ionic events in activation of the mammalian egg. Rev. Reprod. 3, 96–103.CrossRefGoogle ScholarPubMed
Borges, E. Jr, de Almeida Ferreira Braga, D.P., de Sousa Bonetti, T.C., Iaconelli, A. Jr & Franco, J.G. Jr. (2009). Artificial oocyte activation using calcium ionophore in ICSI cycles with spermatozoa from different sources. Reprod. Biomed. Online 18, 45–52.CrossRefGoogle ScholarPubMed
Borini, A., Levi Setti, P.E., Anserini, P., De Luca, R., De Santis, L., Porcu, E., La Sala, G.B., Ferraretti, A., Bartolotti, T., Coticchio, G. & Scaravelli, G. (2010). Multicenter observational study on slow-cooling oocyte cryopreservation: clinical outcome. Fertil. Steril. 94, 1662–8.CrossRefGoogle ScholarPubMed
Chian, R.C., Buckett, W.M., Abdul Jalil, A.K., Son, W.Y., Sylvestre, C., Rao, D. & Tan, S.L. (2004a). Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil. Steril. 82, 1675–8.CrossRefGoogle ScholarPubMed
Chian, R.C., Buckett, W.M. & Tan, S.L. (2004b). In-vitro maturation of human oocytes. Reprod. Biomed. Online 8, 148–66.CrossRefGoogle ScholarPubMed
Cobo, A., Kuwayama, M., Perez, S., Ruiz, A., Pellicer, A. & Remohi, J. (2008). Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil. Steril. 89, 1657–64.CrossRefGoogle ScholarPubMed
Fujinami, N., Hosoi, Y., Kato, H., Matsumoto, K., Saeki, K. & Iritani, A. (2004). Activation with ethanol improves embryo development of ICSI-derived oocytes by regulation of kinetics of MPF activity. J. Reprod. Dev. 50, 171–8.CrossRefGoogle ScholarPubMed
Gidoni, Y., Holzer, H., Tulandi, T. & Tan, S.L. (2008). Fertility preservation in patients with non-oncological conditions. Reprod. Biomed. Online 16, 792–800.CrossRefGoogle ScholarPubMed
Heindryckx, B., Van der Elst, J., De Sutter, P. & Dhont, M. (2005). Treatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI. Hum. Reprod. 20, 2237–41.CrossRefGoogle ScholarPubMed
Hosseini, S.M., Hajian, M., Moulavi, F., Shahverdi, A.H. & Nasr-Esfahani, M.H. (2008). Optimized combined electrical-chemical parthenogenetic activation for in vitro matured bovine oocytes. Anim. Reprod. Sci. 108, 122–33.CrossRefGoogle ScholarPubMed
Huang, J.Y., Tulandi, T., Holzer, H., Tan, S.L. & Chian, R.C. (2008). Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation. Fertil. Steril. 89, 567–72.CrossRefGoogle ScholarPubMed
Jones, A., Van Blerkom, J., Davis, P. & Toledo, A.A. (2004). Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence. Hum. Reprod. 19, 1861–6.CrossRefGoogle ScholarPubMed
Kyono, K., Kumagai, S., Nishinaka, C., Nakajo, Y., Uto, H., Toya, M., Sugawara, J. & Araki, Y. (2008). Birth and follow-up of babies born following ICSI using SrCl2 oocyte activation. Reprod. Biomed. Online 17, 53–8.CrossRefGoogle ScholarPubMed
Lu, Q., Zhao, Y., Gao, X., Li, Y., Ma, S., Mullen, S., Critser, J.K. & Chen, Z.J. (2006). Combination of calcium ionophore A23187 with puromycin salvages human unfertilized oocytes after ICSI. Eur. J. Obstet. Gynecol. Reprod. Biol. 126, 72–6.CrossRefGoogle ScholarPubMed
Nasr-Esfahani, M.H., Razavi, S., Mardani, M., Shirazi, R. & Javanmardi, S. (2007). Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI. Reprod. Biomed. Online 14, 422–9.CrossRefGoogle ScholarPubMed
Nasr-Esfahani, M.H., Razavi, S., Javdan, Z. & Tavalaee, M. (2008). Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection. Fertil. Steril. 90, 2231–7.CrossRefGoogle ScholarPubMed
Ozil, J.P. & Huneau, D. (2001). Activation of rabbit oocytes: the impact of the Ca2+ signal regime on development. Development 128, 917–28.CrossRefGoogle ScholarPubMed
Paynter, S.J. (2000). Current status of the cryopreservation of human unfertilized oocytes. Hum. Reprod. Update 6, 449–56.CrossRefGoogle ScholarPubMed
Tesarik, J., Mendoza, C. & Greco, E. (2000). The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei. Fertil. Steril. 74, 1245–7.CrossRefGoogle ScholarPubMed
Tucker, M.J., Wright, G., Morton, P.C. & Massey, J.B. (1998). Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil. Steril. 70, 578–9.CrossRefGoogle ScholarPubMed
Wu, J., Zhang, L. & Wang, X. (2001). In vitro maturation, fertilization and embryo development after ultrarapid freezing of immature human oocytes. Reproduction 121, 389–93.CrossRefGoogle ScholarPubMed
Zeron, Y., Pearl, M., Borochov, A. & Arav, A. (1999). Kinetic and temporal factors influence chilling injury to germinal vesicle and mature bovine oocytes. Cryobiology 38, 35–42.CrossRefGoogle ScholarPubMed