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Brachyspira spp. and avian intestinal spirochaetosis: an epidemiological review

Published online by Cambridge University Press:  05 September 2013

G.A. MEDHANIE ,
S.A. MCEWEN ,
D. SLAVIC  and
M.T. GUERIN
G.A. MEDHANIE*
Affiliation:
Dept. of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON, N1G 2W1, Canada
S.A. MCEWEN
Affiliation:
Dept. of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON, N1G 2W1, Canada
D. SLAVIC
Affiliation:
Animal Health Laboratory, Laboratory Services Division, University of Guelph, Box 3612, Guelph, ON, N1H 6R8, Canada
M.T. GUERIN
Affiliation:
Dept. of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON, N1G 2W1, Canada
*
Corresponding author: [email protected]
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Abstract

Avian intestinal spirochaetosis (AIS) is primarily a disease of layer and broiler breeder chickens exhibited by delayed and/or reduced egg production, increased mortality, weight loss, and chronic diarrhoea. This disease is of concern to the egg industry because of the economic impact of reduced production and down grading of eggs due to faecal-stained shells. Avian intestinal spirochaetosis is caused by pathogenic Brachyspira spp. This review summarises the species of Brachyspira that have been associated with AIS and provides an overview of epidemiological studies that have investigated prevalence, risk factors, and preventive measures for Brachyspira spp.

Keywords

avian intestinal spirochaetosisBrachyspirapoultrychickensprevalencerisk factors
Type
Review Article
Information
World's Poultry Science Journal , Volume 69 , Issue 3 , September 2013 , pp. 541 - 552
Copyright
Copyright © World's Poultry Science Association 2013

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References

ADACHI, Y., SUEYOSHI, M., MIYAGAWA, E., MINATO, H. and SHOYA, S. (1985) Experimental-infection of young broiler chicks with Treponema hyodysenteriae. Microbiology and Immunology 29: 683-688.CrossRefGoogle ScholarPubMed
BAIT-MERABET, L., THILLE, A., LEGRAND, P., BRUN-BUISSON, C. and CATTOIR, V. (2008) Brachyspira pilosicoli bloodstream infections: Case report and review of the literature. Annals of Clinical Microbiology and Antimicrobials 7: 19.CrossRefGoogle ScholarPubMed
BANO, L., MERIALDI, G., BONILAURI, P., DALL'ANESE, G., CAPELLO, K., COMIN, D., CATTOLI, G., SANGUINETTI, V., HAMPSON, D.J. and AGNOLETTI, F. (2008) Prevalence, disease associations and risk factors for colonization with intestinal spirochaetes (Brachyspira spp.) in flocks of laying hens in north-eastern Italy. Avian Pathology 37: 281-286.CrossRefGoogle ScholarPubMed
BOYE, M., BALODA, S.B., LESER, T.D. and MOLLER, K. (2001) Survival of Brachyspira hyodysenteriae and B. pilosicoli in terrestrial microcosms. Veterinary Microbiology 81: 33-40.Google Scholar
BUCKLES, E.L., EATON, K.A. and SWAYNE, D.E. (1997) Cases of spirochete-associated necrotizing typhlitis in captive common rheas (Rhea americana). Avian Diseases 41: 144-148.Google Scholar
BURCH, D.G.S. (2010) Egg production drops from Brachyspira. http://www.worldpoultry.net/chickens/management/prevention-control/egg-production-drops-from- Brachyspira-7816.html.Google Scholar
BURCH, D.G.S., HARDING, C., ALVAREZ, R. and VALKS, M. (2006) Treatment of a field case of avian intestinal spirochaetosis caused by Brachyspira pilosicoli with tiamulin. Avian Pathology 35: 211-216.CrossRefGoogle ScholarPubMed
CALDERARO, A., BOMMEZZADRI, S., GORRINI, C., PICCOLO, G., PERUZZI, S., VILLANACCI, V., ZAMBELLI, C., DETTORI, G. and CHEZZI, C. (2007) Infective colitis associated with human intestinal spirochetosis. Journal of Gastroenterology and Hepatology 22: 1772-1779.Google Scholar
DAVELAAR, F.G. and SMIT, H.F. (1986) Infectious typhlitis in chickens caused by spirochetes. Avian Pathology 15: 247-258.CrossRefGoogle ScholarPubMed
DUHAMEL, G.E., TROTT, D.J., MUNIAPPA, N., MATHIESEN, M.R., TARASIUK, K., LEE, J.I. and HAMPSON, D.J. (1998) Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated ‘Serpulina canis’ sp. nov. Journal of Clinical Microbiology 36: 2264-2270.Google Scholar
DWARS, R.M., SMIT, H.F. and DAVELAAR, F.G. (1991) Influence of infection with avian intestinal spirochetes on the feces of laying hens. Avian Pathology 21: 513-515.CrossRefGoogle Scholar
DWARS, R.M., SMIT, H.F., DAVELAAR, F.G. and VANTVEER, W. (1989) Incidence of spirochetal infections in cases of intestinal disorder in chickens. Avian Pathology 18: 591-595.CrossRefGoogle ScholarPubMed
FEBERWEE, A., HAMPSON, D.J., PHILLIPS, N.D., LA, T., VAN DER HEIJDEN, H.M., WELLENBERG, G.J., DWARS, R.M. and LANDMAN, W.J.M. (2008) Identification of Brachyspira hyodysenteriae and other pathogenic Brachyspira species in chickens from laying flocks with diarrhea or reduced production or both . Journal of Clinical Microbiology 46: 593-600.CrossRefGoogle ScholarPubMed
FOURNIEAMAZOUZ, E., BARANTON, G., CARLIER, J.P., CHAMBREUIL, G., COHADON, F., COLLIN, P., JOLIVET, A.G., HERMES, I., LEMARIE, C. and SAINTGIRONS, I. (1995) Isolations of intestinal spirochetes from the blood of human patients . Journal of Hospital Infection 30: 160-162.Google Scholar
GLAVITS, R., IVANICS, E., NEMES, C., DAN, A., KASZANYITZKY, E., SAMU, P., THUMA, A., BEREGSZASZI, A., SIMON, A., ALADICS, S., BERTA, P. and DOBOS-KOVACS, M. (2008) Examinations and observations about intestinal spirochaetosis (brachyspirosis) in geese, ducks and domestic fowls, compared to swine dysentery . Magyar Allatorvosok Lapja 130: 663-670.Google Scholar
GRIFFITHS, I.B., HUNT, B.W., LISTER, S.A. and LAMONT, M.H. (1987) Retarded growth-rate and delayed onset of egg-production associated with spirochete infection in pullets . Veterinary Record 121: 35-37.CrossRefGoogle ScholarPubMed
HAMPSON, D.J. and LA, T. (2006) Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov and Brachyspira murdochii comb. nov. International Journal of Systematic and Evolutionary Microbiology 56: 1009-1012.Google Scholar
HAMPSON, D.J., LESTER, G.D., PHILLIPS, N.D. and LA, T. (2006a) Isolation of Brachyspira pilosicoli from weanling horses with chronic diarrhoea . Veterinary Record 158: 661-662.Google Scholar
HAMPSON, D.J. and MCLAREN, A.J. (1999) Experimental infection of laying hens with Serpulina intermedia causes reduced egg production and increased faecal water content . Avian Pathology 28: 113-117.CrossRefGoogle ScholarPubMed
HAMPSON, D.J., OXBERRY, S.L. and LA, T. (2006b) Potential for zoonotic transmission of Brachyspira pilosicoli. Emerging Infectious Diseases 12: 869-870.CrossRefGoogle ScholarPubMed
HAMPSON, D.J., OXBERRY, S.L. and STEPHENS, C.P. (2002a) Influence of in-feed zinc bacitracin and tiamulin treatment on experimental avian intestinal spirochaetosis caused by Brachyspira intermedia. Avian Pathology 31: 285-291.Google Scholar
HAMPSON, D.J., PHILLIPS, N.D. and PLUSKE, J.R. (2002b) Dietary enzyme and zinc bacitracin reduce colonisation of layer hens by the intestinal spirochaete Brachyspira intermedia. Veterinary Microbiology 86: 351-360.CrossRefGoogle ScholarPubMed
HARRIS, D.L., GLOCK, R.D., CHRISTEN, C.R. and KINYON, J.M. (1972) Swine Dysentery .1. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of disease . Veterinary Medicine & Small Animal Clinician 67: 61.Google Scholar
HIRSH, D.C. (2004) Spiral-curved organisms II: Brachyspira (Serpulina), in: HIRSH, D.C., MACLACHLAN, N.J. & WALKER, R.L. (Eds) Veterinary microbiology, 2nd pp. 131-134 (Blackwell, Ames, Iowa).Google Scholar
IVANICS, E., GLAVITS, R., THUMA, A., SIMON, A., BERTA, P., KASZANYITZKY, E., SAMU, P., DENCSO, L., URSU, K. and DAN, A. (2009) Intestinal spirochaetosis (Brachyspirosis) in Hungarian laying hen flocks . Magyar Allatorvosok Lapja 131: 323-330.Google Scholar
JAMSHIDI, A.L., BASSAMI, M.R., KASAEI, A. and MOHAMADI, A. (2009) Isolation and Identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad-Iran. The 6th National Biotechnology Congress of Iran, Tehran-Iran.Google Scholar
JAMSHIDI, A. and HAMPSON, D.J. (2005) The eggs from laying hens infected with Brachyspira pilosicoli may not be a potential source of infection for human . Iranian Journal of Veterinary Research 6: 1-7.Google Scholar
JAMSHIDI, A. and HAMPSON, D.J. (2003) Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli. Journal of Medical Microbiology 52: 361-364.CrossRefGoogle Scholar
JAMSHIDI, A. and HAMPSON, D.J. (2002) Zinc bacitracin enhances colonization by the intestinal spirochaete Brachyspira pilosicoli in experimentally infected layer hens . Avian Pathology 31: 293-298.CrossRefGoogle ScholarPubMed
JAMSHIDIAN, M., LA, T., PHILLIPS, N.D. and HAMPSON, D.J. (2004) Brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation . Journal of Medical Microbiology 53: 313-318.CrossRefGoogle ScholarPubMed
JANSSON, D.S., FELLSTROM, C. and JOHANSSON, K.E. (2008) Intestinal spirochetes isolated from wild-living jackdaws, hooded crows and rooks (genus Corvus): Provisionally designated ‘Brachyspira corvi’ sp nov. Anaerobe 14: 287-295.Google Scholar
JANSSON, D.S., BROJER, C., GAVIER-WIDEN, D., GUNNARSSON, A. and FELLSTROM, C. (2001) Brachyspira spp. (Serpulina spp.) in birds: A review and results from a study of Swedish game birds . Animal Health Research Reviews 2: 93-100.CrossRefGoogle ScholarPubMed
JENSEN, N.S., STANTON, T.B. and SWAYNE, D.E. (1996) Identification of the swine pathogen Serpulina hyodysenteriae in rheas (Rhea americana) . Veterinary Microbiology 52: 259-269.Google Scholar
JENSEN, T.K., CHRISTENSEN, A.S. and BOYE, M. (2010) Brachyspira murdochii colitis in pigs. Veterinary Pathology 47: 334-338.CrossRefGoogle ScholarPubMed
JOENS, L.A. (1980) Experimental Transmission of Treponema hyodysenteriae from mice to pigs . American Journal of Veterinary Research 41: 1225-1226.Google Scholar
JOENS, L.A. and KINYON, J.M. (1982) Isolation of Treponema hyodysenteriae from wild rodents . Journal of Clinical Microbiology 15: 994-997.Google Scholar
JORDAN, F.T. and HAMPSON, D.J. (2008) Brachyspira spp. Intestinal Spirochaetosis, in: PATTISON, M., MCMULLIN, P., BRADBURY, J.M. & ALEXANDER, D.J. (Eds) Poultry Diseases, pp. 243-247 (Elsevier Philadelphia, USA).Google Scholar
KINYON, J.M. and HARRIS, D.L. (1979) Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae Harris et al . International Journal of Systematic Bacteriology 29: 102-109.CrossRefGoogle Scholar
KIZERWETTER-ŚWIDA, M., RZEWUSKA, M. and BINEK, M. (2005) Characterization of Brachyspira sp strains isolated from flock of hens with diarrhoea . Bulletin of the Veterinary Institute in Pulawy 49: 169-173.Google Scholar
LAZARO, R., GARCIA, M., ARANIBAR, M.J. and MATEOS, G.G. (2003) Effect of enzyme addition to wheat-, barley- and rye-based diets on nutrient digestibility and performance of laying hens. British Poultry Science 44: 2.Google Scholar
LUX, R., MOTER, A. and SHI, W.Y. (2000) Chemotaxis in pathogenic spirochetes: Directed movement toward targeting tissues? Journal of Molecular Microbiology and Biotechnology 2: 355-364.Google ScholarPubMed
MCLAREN, A.J., HAMPSON, D.J. and WYLIE, S.L. (1996) The prevalence of intestinal spirochaetes in poultry flocks in Western Australia . Australian Veterinary Journal 74: 319-321.Google Scholar
MEDHANIE, G.A., MCEWEN, S.A., WEBER, L., SANEI, B., COOLEY, L., HOUGHTON, S., SLAVIC, D. and GUERIN, M.T. (2013) Risk factors associated with the colonization of Ontario layer chicken flocks with Brachyspira species. Preventive Veterinary Medicine 109: 304-311.Google Scholar
MEDHANIE, G.A., MCEWEN, S.A., WEBER, L., SANEI, B., COOLEY, L., HOUGHTON, S., SLAVIC, D. and GUERIN, M.T. (2013) Brachyspira species in Ontario layer chicken flocks and its association with downgrades for dirty eggs. Manuscript in preparation for submission to Avian Diseases.Google Scholar
MIKOSZA, A.S.J. and HAMPSON, D.J. (2001) Human intestinal spirochetosis: Brachyspira aalborgi and/or Brachyspira pilosicoli? Animal Health Research Reviews 2: 101-110.CrossRefGoogle ScholarPubMed
MUNSHI, M.A., TAYLOR, N.M., MIKOSZA, A.S.J., SPENCER, P.B.S. and HAMPSON, D.J. (2003) Detection by PCR and isolation assays of the anaerobic intestinal spirochete Brachyspira aalborgi from the feces of captive nonhuman primates . Journal of Clinical Microbiology 41: 1187-1191.Google Scholar
MYERS, S.E., DUNN, P.A., PHILLIPS, N.D., LA, T. and HAMPSON, D.J. (2009) Brachyspira intermedia and Brachyspira pilosicoli are commonly found in older laying flocks in Pennsylvania . Avian Diseases 53: 533-537.CrossRefGoogle ScholarPubMed
NEMES, C.S., GLAVITS, R., DOBOS-KOVACS, M., LVANICS, T., KASZANYITZKY, E., BEREGSZASZI, A., SZEREDI, L. and DENCSO, L. (2006) Typhlocolitis associated with spirochaetes in goose flocks . Avian Pathology 35: 4-11.CrossRefGoogle ScholarPubMed
OCHIAI, S., ADACHI, Y. and MORI, K. (1997) Unification of the genera Serpulina and Brachyspira, and proposals of Brachyspira hyodysenteriae comb nov, Brachyspira innocens comb nov and Brachyspira pilosicoli comb nov . Microbiology and Immunology 41: 445-452.Google Scholar
OXBERRY, S.L. and HAMPSON, D.J. (2003) Colonisation of pet shop puppies with Brachyspira pilosicoli. Veterinary Microbiology 93: 167-174.Google Scholar
OXBERRY, S.L., TROTT, D.J. and HAMPSON, D.J. (1998) Serpulina pilosicoli, waterbirds and water: potential sources of infection for humans and other animals . Epidemiology and Infection 121: 219-225.CrossRefGoogle ScholarPubMed
PASTER, B.J. and DEWHIRST, F.E. (2000) Phylogenetic foundation of spirochetes . Journal of Molecular Microbiology and Biotechnology 2: 341-344.Google Scholar
PHILLIPS, N.D., LA, T. and HAMPSON, D.J. (2005) A cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (Brachyspira spp.) in three flocks of laying hens . Veterinary Microbiology 105: 189-198.Google Scholar
PHILLIPS, N.D., LA, T. and HAMPSON, D.J. (2003) Survival of intestinal spirochaete strains from chickens in the presence of disinfectants and in faeces held at different temperatures . Avian Pathology 32: 639-643.Google Scholar
PHILLIPS, N.D., LA, T., PLUSKE, J.R. and HAMPSON, D.J. (2004) The wheat variety used in the diet of laying hens influences colonization with the intestinal spirochaete Brachyspira intermedia. Avian Pathology 33: 586-590.CrossRefGoogle ScholarPubMed
RÅSBÄCK, T., JANSSON, D.S., JOHANSSON, K.E. and FELLSTROM, C. (2007) A novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated 'Brachyspira suanatina' sp nov. Environmental Microbiology 9: 983-991.Google Scholar
RUANE, P.J., NAKATA, M.M., REINHARDT, J.F. and GEORGE, W.L. (1989) Spirochete like organisms in the human gastrointestinal tract . Reviews of infectious diseases 11: 184-196.Google Scholar
SHIBAHARA, T., HIKITA, M., WADA, Y., SUEYOSHI, M., OHYA, T., ISHIKAWA, Y. and KADOTA, K. (2000a) Intestinal spirochetosis in wild sika deer (Cervus nipponyesoensis) infected with Brachyspira species . Journal of Veterinary Medical Science 62: 947-951.Google Scholar
SHIBAHARA, T., KUWANO, A., UENO, T., ANZAI, T., KUWAMOTO, Y., SATO, H., MAEDA, T., ISHIKAWA, Y. and KADOTA, K. (2002a) Intestinal spirochetosis in a 21-month-old Thoroughbred colt . Journal of Veterinary Medical Science 64: 633-636.Google Scholar
SHIBAHARA, T., OHYA, T., ISHII, R., OGIHARA, Y., MAEDA, T., ISHIKAWA, Y. and KADOTA, K. (2002b) Concurrent spirochaetal infections of the feet and colon of cattle in Japan . Australian Veterinary Journal 80: 497-502.CrossRefGoogle ScholarPubMed
SHIBAHARA, T., WADA, Y., SUEYOSHI, M., OHYA, T., ISHIKAWA, Y. and KADOTA, K. (2000b) Bovine intestinal spirochaetosis with dysentery . Veterinary Record 146: 585-586.Google Scholar
SHIVAPRASAD, H.L. and DUHAMEL, G.E. (2005) Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys . Avian Diseases 49: 609-613.CrossRefGoogle ScholarPubMed
SMITH, J.L. (2005) Colonic spirochetosis in animals and humans . Journal of Food Protection 68: 1525-1534.CrossRefGoogle Scholar
SONGER, J.G. and HARRIS, D.L. (1978) Transmission of Swine-dysentery by carrier pigs . American Journal of Veterinary Research 39: 913-916.Google Scholar
STANTON, T.B., FOURNIEAMAZOUZ, E., POSTIC, D., TROTT, D.J., GRIMONT, P.A.D., BARANTON, G., HAMPSON, D.J. and SAINTGIRONS, I. (1997) Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. International Journal of Systematic Bacteriology 47: 1007-1012.Google Scholar
STANTON, T.B., POSTIC, D. and JENSEN, N.S. (1998) Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens . International Journal of Systematic Bacteriology 48: 669-676.Google Scholar
STEGE, H., JENSEN, T.K., MOLLER, K., BAEKBO, P. and JORSAL, S.E. (2001) Risk factors for intestinal pathogens in Danish finishing pig herds. Preventive Veterinary Medicine 50: 153-164.Google Scholar
STEPHENS, C.P. and HAMPSON, D.J. (2002a) Evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete Brachyspira pilosicoli. Avian Pathology 31: 299-304.Google Scholar
STEPHENS, C.P. and HAMPSON, D.J. (2002b) Experimental infection of broiler breeder hens with the intestinal spirochaete Brachyspira (Serpulina) pilosicoli causes reduced egg production . Avian Pathology 31: 169-175.Google Scholar
STEPHENS, C.P. and HAMPSON, D.J. (2001) Intestinal spirochete infections of chickens: A review of disease associations, epidemiology and control . Animal Health Research Reviews 2: 83-91.Google Scholar
STEPHENS, C.P. and HAMPSON, D.J. (1999) Prevalence and disease association of intestinal spirochaetes in chickens in eastern Australia . Avian Pathology 28: 447-454.Google Scholar
SWAYNE, D.E., BERMUDEZ, A.J., SAGARTZ, J.E., EATON, K.A., MONFORT, J.D., STOUTENBURG, J.W. and HAYES, J.R. (1992) Association of cecal spirochetes with pasty vents and dirty eggshells in layers . Avian Diseases 36: 776-781.CrossRefGoogle ScholarPubMed
SWAYNE, D.E., EATON, K.A., STOUTENBURG, J., TROTT, D.J., HAMPSON, D.J. and JENSEN, N.S. (1995) Identification of a new intestinal spirochete with pathogenicity for chickens . Infection and Immunity 63: 430-436.Google Scholar
TAYLOR, D.J. (1997) Swine dysentery . Magyar Allatorvosok Lapja 119: 612-615.Google Scholar
TRIVETT-MOORE, N.L., GILBERT, G.L., LAW, C.L.H., TROTT, D.J. and HAMPSON, D.J. (1998) Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis . Journal of Clinical Microbiology 36: 261-265.Google Scholar
TROTT, D.J., COMBS, B.G., MIKOSZA, A.S.J., OXBERRY, S.L., ROBERTSON, I.D., PASSEY, M., TAIME, J., SEHUKO, R., ALPERS, M.P. and HAMPSON, D.J. (1997) The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea . Epidemiology and Infection 119: 369-379.CrossRefGoogle ScholarPubMed
TROTT, D.J., HUXTABLE, C.R. and HAMPSON, D.J. (1996a) Experimental infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli. Infection and Immunity 64: 4648-4654.Google Scholar
TROTT, D.J., MCLAREN, A.J. and HAMPSON, D.J. (1995) Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks - an animal-model of intestinal spirochetosis . Infection and Immunity 63: 3705-3710.CrossRefGoogle ScholarPubMed
TROTT, D.J., STANTON, T.B., JENSEN, N.S., DUHAMEL, G.E., JOHNSON, J.L. and HAMPSON, D.J. (1996b) Serpulina pilosicoli sp nov; The agent of porcine intestinal spirochetosis . International Journal of Systematic Bacteriology 46: 206-215.CrossRefGoogle ScholarPubMed
VERLINDEN, M., PASMANS, F., GARMYN, A., DE ZUTTER, L., HAESEBROUCK, F. and MARTEL, A. (2012) Occurrence of viable Brachyspira spp. on carcasses of spent laying hens from supermarkets. Food Microbiology 32: 321-324.CrossRefGoogle ScholarPubMed
WEISSENBÖCK, H., MADERNER, A., HERZOG, A.M., LUSSY, H. and NOWOTNY, N. (2005) Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspira murdochii. Veterinary Microbiology 111: 67-75.Google Scholar