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Synaptic connections involving immunoreactive glycine receptors in the turtle retina

Published online by Cambridge University Press:  02 June 2009

Charles L. Zucker  and
Berndt Ehinger
Charles L. Zucker
Affiliation:
Schepens Eye Research Institute and Harvard Medical School, Department of Ophthalmology, Boston
Berndt Ehinger
Affiliation:
Department of Ophthalmology, University of Lund, Lund, Sweden
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Abstract

The distribution of glycine receptors in the turtle retina was studied with the aid of a monoclonal antibody that detects the 93-kD protein associated with the strychnine-sensitive glycine receptor. Light microscopically, receptors were found in the inner plexiform layer and, more sparsely, in the innermost parts of the inner nuclear layer. No receptors were seen to be associated with photoreceptor cells, horizontal cells, or any other structures in the distal inner nuclear layer or outer plexiform layer. Ultrastructurally, glycine receptors were found on the inner face of postsynaptic membranes of processes from amacrine and presumed ganglion cells and always involved amacrine cell processes as the presynaptic element. Such glycine receptor immunoreactive synapses onto amacrine cell processes were distributed throughout the inner plexiform layer with a peak density near the middle. On the other hand, output synapses onto ganglion cell processes displaying immunoreactive glycine receptor sites showed a bimodal distribution in the inner plexiform layer. Glycine receptor immunoreactivity was not detected on bipolar cells, but presumed glycine-utilizing processes (i.e. those presynaptic to immunoreactive glycine receptors) were occasionally found to be postsynaptic in bipolar cell dyads. The majority of the synaptic input to the presumed glycine-utilizing amacrine cell processes was from other amacrine processes, some of which were themselves glycine utilizing. The observations suggest that glycinergic synapses in the turtle retina are, to a large extent, engaged in processing interamacrine signals.

Keywords

Strychnine-sensitive glycine receptorsAmacrine cellsImmunohistochemistryElectron microscopyTurtle retina
Type
Research Articles
Information
Visual Neuroscience , Volume 10 , Issue 5 , September 1993 , pp. 907 - 914
Copyright
Copyright © Cambridge University Press 1993

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