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Yeast cell metabolism investigated by CO2 production and soft X-ray irradiation

Published online by Cambridge University Press:  15 January 1999

A. Masini ,
D. Batani ,
F. Previdi ,
M. Milani ,
A. Pozzi ,
E. Turcu ,
S. Huntington  and
H. Takeyasu
A. Masini
Affiliation:
Dipartimento di Fisica, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy INFM, Istituto Nazionale di Fisica della Materia, Milano, Italy
D. Batani
Affiliation:
Dipartimento di Fisica, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy INFM, Istituto Nazionale di Fisica della Materia, Milano, Italy
F. Previdi
Affiliation:
INFM, Istituto Nazionale di Fisica della Materia, Milano, Italy Dipartimento di Elettronica e Informazione, Politecnico di Milano, via Ponzio 34/5, 20133 Milano, Italy
M. Milani
Affiliation:
INFM, Istituto Nazionale di Fisica della Materia, Milano, Italy Dipartimento di Scienza dei Materiali, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy
A. Pozzi
Affiliation:
Dipartimento di Fisica, Seconda Università di Milano Bicocca, via Emanueli 15, 20126 Milano, Italy
E. Turcu
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, Oxon, OX11 OQX, UK
S. Huntington
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, Oxon, OX11 OQX, UK
H. Takeyasu
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, Oxon, OX11 OQX, UK
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Abstract

Results obtained using a new technique for studying cell metabolism arepresented. The technique, consisting in CO2 production monitoring, has been appliedto Saccharomyces cerevisiae yeast cells. Also the cells were irradiated using thesoft X-ray laser-plasma source at Rutherford Appleton Laboratory with the aim of producing a damage of metabolic processes at the wall level, responsible forfermentation, without great interference with respiration, taking place inmitochondria, and DNA activity. The source was calibrated with PIN diodes and X-rayspectrometers and used Teflon stripes as target, emitting X-rays at about 0.9 keV, witha very low penetration in biological material. X-ray doses delivered to the differentcell compartments were calculated following a Lambert-Bouguet-Beer law. Immediatelyafter irradiation, the damage to metabolic activity was measured again by monitoringCO2 production. Results showed a general reduction in gas production by irradiatedsamples, together with non-linear and non-monotone response to dose. There was alsoevidence of oscillations in cell metabolic activity and of X-ray induced changes inoscillation frequency.

Keywords

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 5 , Issue 1 , January 1999 , pp. 101 - 109
Copyright
© EDP Sciences, 1999

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