We have obtained a longslit spectrum at a position angle (PA) of 84.6° and passing within 0.38 arcsec of the nucleus of NGC 4151, using the FOC f/48 camera on the Hubble Space Telescope. The spectrum shows strong emission lines including [OII] λ 3727 and [OIII] λλ 4959, 5007. By fitting with Gaussian velocity profiles, we resolve the emission lines, within 1 arcsec of the nucleus, into a high and low velocity component. The low velocity component has a total range in radial velocity of 200 km s−1 and appears to be associated with material comprising the knots seen in the FOC, F501N [O III] image of NGC 4151, illustrated in Boksenberg (1993). The much weaker high velocity system has a range of 1000 km s−1, is more smoothly distributed in brightness and shows a peak brightness close to the nucleus. Because the slit did not intersect the nucleus it is possible to determine the PA at which the two velocity systems cross the zero velocity axis. This is at PA −26° for the low velocity system and PA +32° for the high velocity system. These PA values may be subject to a systematic error as the zero velocity is defined by the mean position of the line, in the absence of any external calibration.