Kinetic transfer coefficients are important parameters to understand and reliably model the behaviour of non-conservative radionuclides in aquatic environments. This report pertains to a series of radiotracer experiments on 239Pu uptake in natural unfiltered waters from the Hueznar reservoir (southern Spain). The experimental procedure ensures the preservation of environmental conditions and the dissolved Pu activity was measured by liquid scintillation technique. The data suggest that the main pathways for Pu uptake consist of two parallel and reversible reactions. We studied the effects of suspended matter concentrations and the specific surface area on the kinetic transfer coefficients.