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Induction of pathogen defence genes in parsley (Petroselinum crispum L.) plants by ozone

Published online by Cambridge University Press:  05 December 2011

Heidrun Eckey-Kaltenbach ,
Erich Großkopf ,
Heinrich Sandermann Jr  and
Dietrich Ernst
Heidrun Eckey-Kaltenbach
Affiliation:
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, D-85764 Oberschleißheim, Germany
Erich Großkopf
Affiliation:
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, D-85764 Oberschleißheim, Germany
Heinrich Sandermann Jr
Affiliation:
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, D-85764 Oberschleißheim, Germany
Dietrich Ernst*
Affiliation:
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, D-85764 Oberschleißheim, Germany
*
§Author for proofs and correspondence (fax +49 89 3187 3383).
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Synopsis

Parsley (Petroselinum crispum L.) is known to respond to pathogen attack by the synthesis of furanocoumarins and to UV-irradiation by the synthesis of flavone glycosides, whereas ozone treatment results in the induction of both pathways. Ozone treatment (200 nl 1−1, 10 h) of parsley seedlings results in an increased mRNA level of early genes within 3 h [pathogenesis related proteins PR1, PR2 and an elicitor-induced protein with unknown function (Eli 16)], of intermediate induced genes within 6 h [phenylalanine ammonia-lyase (PAL), 4-coumaroyl-CoA ligase (4CL), chalcone synthase (CHS)], and of late genes within 12 h [hydroxyproline-rich glycoprotein (HRGP), peroxidase (POD)]. 2D-PAGE of in vitro translated poly(A)+ RNA isolated from ozone-treated parsley seedlings revealed about 20 induced and 10 repressed translation products. A cDNA library from parsley seedlings was differential screened, yielding several induced cDNA clones. One of the ozone-induced cDNA clones could be identified as coding for PR1-1 by hybrid-selected in vitro translation and by DNA sequence analysis.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 63 - 74
Copyright
Copyright © Royal Society of Edinburgh 1994

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Footnotes

*Present address: Amersham Buchler, Gieselweg 1, Postfach 1149, D-38110 Braunschweig, Germany.

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