Previous studies on glucosinolate (GS) toxicity have focused on progoitrin since it is the most abundant GS in single-low varieties of oilseed rape. Progoitrin levels have been greatly reduced in new double-low varieties, but levels of indole GS (glucobrassicin and 4-hydroxy glucobrassicin) have remained relatively static and represent a much higher proportion of total residual levels. In this study, the aim was to evaluate the metabolic and toxic effects of rapeseed differing in indole GS and progoitrin concentration.