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Protein degradation kinetics of un- and xylose treated soya bean meal by using SDS-PAGE

Published online by Cambridge University Press:  20 November 2017

A.A. Sadeghi
Affiliation:
Department of Animal Science, Science & Research Branch, Islamic Azad University, Tehran, Iran.
A. Nikkhah
Affiliation:
Department of Animal Science, Science & Research Branch, Islamic Azad University, Tehran, Iran.
M.M. Shahrebabak
Affiliation:
Department of Animal Science, Science & Research Branch, Islamic Azad University, Tehran, Iran.
P. Shawrang
Affiliation:
Department of Animal Science, Science & Research Branch, Islamic Azad University, Tehran, Iran.
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Extract

High producing or rapidly growing ruminants require more high quality protein than is provided by rumen microorganisms. This protein can be supplied by increasing the amount of dietary protein escaping degradation in the rumen. Solvent-extracted soya bean meal (SBM), which is a commonly used protein supplement to high producing dairy cows, is palatable with a well-balanced amino acid composition. However, SBM proteins are extensively degraded in the rumen. Attempts to decrease the rate and extent of ruminal degradation of soya bean meal proteins involved treatment with physical and chemical agents. Recently Tuncer and Sacakli (2003) reported that xylose reduce soya bean meal crude protein (CP) degradation in the rumen. To our knowledge, little information is available concerning the type of proteins of un- and xylose treated soya bean meal that leaves the rumen undegraded. The main objectives of our research were to evaluate the degradation of un- and xylose treated soya bean meal proteins by using SDS-PAGE and determine Effective Rumen Degradation (ERD) and intestinal digestibility of un- and xylose treated soya bean meal CP.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2004

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References

Calsamiglia, S., Stern, M.D. 1995. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. Journal of Animal Science 73: 1459.CrossRefGoogle ScholarPubMed
Laemmli, U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature. 227: 680.CrossRefGoogle ScholarPubMed
Ørskov, E.R., McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. Camb. 92: 499503.CrossRefGoogle Scholar
Tuncer, S.D. and Sacakli, P.. 2003. Rumen degradability characteristics of xylose treated canola and soybean meals. Animal Feed Science and Technology 107: 211–218.CrossRefGoogle Scholar