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Expression of igf-i and gh-receptor (ghr) mrna in liver and skeletal muscle of different pig breeds

Published online by Cambridge University Press:  20 November 2017

J.M. Brameld ,
J.L. Atkinson ,
T.J. Budd ,
J.C. Saunders ,
A.M. Salter ,
R.S. Gilmour  and
P.J. Buttery
J.M. Brameld
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
J.L. Atkinson
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
T.J. Budd
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
J.C. Saunders
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge, CB2 4AT
A.M. Salter
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
R.S. Gilmour
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge, CB2 4AT
P.J. Buttery
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
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Extract

Previous studies of the porcine GH-IGF axis have demonstrated age related changes in liver, but not LD muscle, in expression of both GH-receptor (GHR) and IGF-I genes (Brameld et al, 1993; 1995), and also effects of energy availability on the expression of IGF-I in liver, but not in LD muscle (Weller et al, 1994). The work described here was carried out to study the expression of IGF-I and GHR mRNA in liver and skeletal muscle from different breeds of pig. The probes used were as described previously (Brameld et al, 1993; 1995; Weller et al, 1993; 1994), and were designed to monitor promoter usage for IGF-I expression, with the two promoters giving rise to class 1 and 2 transcripts, and also expression of the extra-cellular domain of the GH-receptor.

Type
Posters for Theatre Session
Information
Proceedings of the British Society of Animal Science , Volume 1995 , March 1995 , pp. 183
Copyright
Copyright © The British Society of Animal Science 1995

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References

Brameld, J.M., Buttery, P.J., Weller, P.A., & Gilmour, R.S. (1993). Ontogenic study of expression of igf-i and gh-receptor (ghr) mrna in pig liver and skeletal muscle (abstract). Animal Production 56: 457.Google Scholar
Brameld, J.M., Weller, P.A., Pell, J.M., Buttery, P.J., & Gilmour, R.S. (1995). Ontogenic study of insulin-like growth factor-I and growth hormone receptor mRNA expression in porcine liver and skeletal muscle. Submitted to Animal Science. 10.1017/S1357729800013886CrossRefGoogle Scholar
Chomczynski, P., & Sacchi, N. (1989). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162: 156–159.10.1016/0003-2697(87)90021-2CrossRefGoogle Scholar
Pell, J.M., Saunders, J.C., & Gilmour, R.S. (1993). Differential regulation of transcription initiation from insulin-like growth factor-i (igf-i) leader exons and of tissue igf-i expression in response to changed growth hormone and nutritional status in sheep. Endocrinology 132: 1797–1807.10.1210/endo.132.4.8462477CrossRefGoogle ScholarPubMed
Weller, P.A., Dickson, M.C., Huskisson, N.S., Dauncey, M.J., Buttery, P.J., & Gilmour, R.S. (1993). The porcine insulin-like growth factor-i gene: characterization and expression of alternate transcription sites. Journal of Molecular Endocrinology 11: 201–211.10.1677/jme.0.0110201CrossRefGoogle ScholarPubMed
Weller, P.A., Dauncey, M.J., Bates, P.C., Brameld, J.M., Buttery, P.J., & Gilmour, R.S. (1994). Regulation of porcine insulin-like growth factor-i and growth hormone receptor mrna expression by energy status. American Journal of Physiology 266: E776–E785.Google ScholarPubMed