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Identification of quantitative trait loci associated with soybean seed protein content using two populations derived from crosses between Glycine max and Glycine soja

Published online by Cambridge University Press:  16 July 2014

Long Yan ,
Li-Li Xing ,
Chun-Yan Yang ,
Ru-Zhen Chang ,
Meng-Chen Zhang  and
Li-Juan Qiu
Long Yan
Affiliation:
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang Branch Center of National Center for Soybean Improvement, The Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, People's Republic of China
Li-Li Xing
Affiliation:
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China
Chun-Yan Yang
Affiliation:
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang Branch Center of National Center for Soybean Improvement, The Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, People's Republic of China
Ru-Zhen Chang
Affiliation:
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China
Meng-Chen Zhang
Affiliation:
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang Branch Center of National Center for Soybean Improvement, The Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, People's Republic of China
Li-Juan Qiu*
Affiliation:
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China
*
* Corresponding author. E-mail: [email protected]
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Abstract

Seed protein content is one of the most important traits controlled by quantitative trait loci (QTLs) in soybean. In this study, a Glycine soja accession (ZYD2738) was crossed with two elite cultivars Jidou 12 and Jidou 9 separately and subsequently the resulting F2:3 populations were used to identify QTLs associated with seed protein content. Protein contents in either population appeared to have a normal distribution with transgressive segregation. A total of five QTLs associated with high protein content were identified and mapped to chromosomes 2, 6, 13, 18 and 20, respectively. Of these QTLs, three (qPRO_2_1, qPRO_13_1 and qPRO_20_1) were identified in the same region in both the populations, whereas the other two (qPRO_6_1 and qPRO_18_1) were mapped in two different regions. qPRO_2_1 appears to be a novel protein QTL. qPRO_6_1, qPRO_18_1 and qPRO_20_1 had additive effects on seed protein content, while qPRO_13_1 had an over-dominant effect on seed protein content. These QTLs and their linked markers could serve as effective tools for marker-assisted selection to increase seed protein content.

Keywords

Glycine maxGlycine sojaQTLsseed protein content
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S104 - S108
Copyright
Copyright © NIAB 2014 

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