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Implementation of BioMEMS for Determining Mechanical Properties of Biological Cells

Published online by Cambridge University Press:  12 January 2012

Svetlana Tatic-Lucic  and
Markus Gnerlich
Svetlana Tatic-Lucic
Affiliation:
ECE Department, Lehigh University, Bethlehem, PA, 18015, U.S.A.
Markus Gnerlich
Affiliation:
ECE Department, Lehigh University, Bethlehem, PA, 18015, U.S.A.
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Abstract

This paper describes the implementation of a custom-made bio-microelectromechanical system for determining mechanical properties of biological cells, which is used for the measurement of mechanical properties of fibroblasts. Our system consists of several subcomponents: (a) actuator which deforms the cell in pre-determined, step-wise fashion, (b) force sensor that measures force applied onto the cell, (c) set of dielectrophoretic (DEP) electrodes for positioning cells in the desired position, (d) temperature sensors and (e) heater. Preliminary results of the mechanical properties of NIH3T3 cells have been determined using this tool and our cell compression techniques.

Keywords

microelectro-mechanical (MEMS)biomedicalsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii05-01
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

1. Moraes, C., Sun, Y., Simmons, C.A., “Microfabricated Devices for Studying Cellular Biomechanics and Mechanobiology,” Cellular and Biomolecular Mechanics and Mechanobiology, ed. Gefen, A. (Springer, 2011) pp. 145–175.Google Scholar
2. Rajagopalan, R., Taher, M. Saif, A., “MEMS sensors and microsystems for cell mechanobiology,” J. Micromechanics and Microengineering 21, 054002 (2011).Google Scholar
3. Kim, D.H., Kin Wong, P., Park, J., Levchenko, A., Sun, Y., “Microengineered platforms for cell mechanobiology,” Annual Reviews of Biomedical Engineering 11, 203–233 (2009).Google Scholar
4. Ingber, Donald, “Mechanobiology and diseases of mechanotransduction,” Annals of Medicine 35, 564–577 (2003).Google Scholar
5. Lincoln, B., Schinkinger, S., Travis, K., Wottawah, F., Ebert, S., Sauer, F., Guck, J., “Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications,” Biomedical Microdevices 9, 703–710 (2007).Google Scholar
6. Rosenbluth, M.J., Lam, W.A., Fletcher, D.A.. “Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry,” Lab on a Chip 8, 1062–1070 (2008).Google Scholar
7. Chen, J., Zheng, Y., Tan, Q., Shojaei-Baghini, E., Zhang, Y.L., Li, J., Prasad, P., You, L., Wu, X.Y., Sun, Y., “Classification of cell types using a microfluidic device for mechanical and electrical measurement on single cells,” Lab on a Chip 11, 3174–3181 (2011).Google Scholar
8. Eklund, E. J., Shkel, A.M., “Single-mask fabrication of high-G piezoresistive accelerometers with extended temperature range,” J. Micromechanics and Microengineering 17, 730–736 (2007).Google Scholar
9. Gnerlich, M., Perry, S.F., Tatic-Lucic, S.. “A Submersible Piezoresistive MEMS Lateral Force Sensor for Cellular Biomechanics Applications,” The 16th International Conference on Solid-State Sensors, Actuators and Microsystems, (IEEE, Beijing, 2011). pp. 2207–2210.Google Scholar
10. Gnerlich, M.. Ph.D. dissertation, “Microelectromechanical Actuator and Sensor System for Measuring the Mechanical Compliance of Biological Cells,” Lehigh University, 2011.Google Scholar
11. Sounart, T., Michalske, T., Zavadil, K., “Frequency-Dependent Electrostatic Actuation in Microfluidic MEMS,” J. of Microelectromechanical Systems 14, 125–133 (2005).Google Scholar
12. Mukundan, V., Pruitt, B., “MEMS Electrostatic Actuation in Conducting Biological Media.” J. Microelectromechanical Systems 18, 405–413 (2009).Google Scholar
13. Timoshenko, S.P., Goodier, J.N., Theory of Elasticity, 3rd ed. (McGraw-Hill Publishing Company, 1970) pp. 409–414.Google Scholar
14. Lu, W.M., Tunga, K.L., Hunga, S.M., Shiaua, J.S., Hwang, K.J., “Compression of deformable gel particles,” Powder Technology 116, 1–12 (2001).Google Scholar
15. Peeters, E., Oomens, C., Bouten, C., Bader, D., Baaijens, F., “Viscoelastic Properties of Single Cells Under Compression,” J. Biomechanical Engineering 127, 237–243 (2005).Google Scholar
16. Roylance, D., Mechanics of Materials, (John Wiley & Sons, 1996).Google Scholar
17. Thoumine, O., Ott, A., “Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation,” J. Cell Science 110, 2109–2116 (1997).Google Scholar