Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T02:11:22.424Z Has data issue: false hasContentIssue false
  • English
  • Français

Fluorescence Measurements and AFM Imaging of Bacteriorhodopsin Coupled with CdSe Quantum Dots for Optoelectronic Applications

Published online by Cambridge University Press:  31 January 2011

Nicolas Bouchonville ,
Michael Molinari ,
Alyona Sukhanova ,
Michel Troyon  and
Igor Nabiev
Nicolas Bouchonville
Affiliation:
[email protected], URCA-LMEN, physics, reims, France
Michael Molinari
Affiliation:
[email protected]@hotmail.com
Alyona Sukhanova
Affiliation:
[email protected], URCA-DAT, biochemistry, reims, France
Michel Troyon
Affiliation:
[email protected], URCA-LMEN, physics, reims, France
Igor Nabiev
Affiliation:
[email protected], URCA-DAT, biochemistry, reims, France
Get access

Abstract

A new nanohybrid material with a potential impact on energy transfer processes in biomolecules was developed by coupling colloidal fluorescent semiconductor CdSe/ZnS quantum dots (QDs) with a photochromic membrane protein, the bacteriorhodopsin (bR). The interactions between the nanocrystals and the proteins were studied by fluorescence spectroscopy and atomic force microscopy (AFM) measurements. A quenching in the photoluminescence (PL) of QDs emitting in the range of the bR absorption suggests a fluorescence resonance energy transfer effect from QDs (donors) to bR (acceptor). As the quenching evolution is different with the surface charges of the QDs, it suggests that the QDs interact with bR through electrostatic interactions. The AFM images of bR coupled with QDs capped with positive or negative surface groups confirm that the electrostatic interactions between QDs and bR play a dominant role in the way they are coupling together. The observed interactions between QDs and bR can provide the basis for the development of novel functional materials with unique photonic properties and having applications in the all-optical switching, photovoltaics and data storage.

Keywords

biomaterialnanostructureoptical properties
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1237: Symposium TT – Nanobiotechnology and Nanobiophotonics - Opportunities and Challenges , 2009 , 1237-TT10-03
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Boxer, S. G. Stocker, J. Franzen, S. Salafsky, J. “Re-engineering photosynthetic reaction centers”, Molecular Electronics-Science and Technology, ed. Aviram, A. (American Institute of Physics, 1992) 262, 226–241.Google Scholar
2 Lee, I. Lee, J. W. Greenbaum, E. Phys. Rev. Lett. 79, 3294–3297 (1997).Google Scholar
3 Hampp, N. A. Chem. Rev. 100, 1755 (2000).Google Scholar
4 Niemeyer, C. Mirkin, C. Nanobiology: Concepts, Applications and Perspectives (Wiley) 146 (2004).Google Scholar
5 Hampp, N. A. Appl. Microbiol. Biotechnol. 53, 633–639 (2000).Google Scholar
6 Nabiev, I. Efremov, R. G. Chumanov, G. D. J. Biosciences 8, 363–373 (1985).Google Scholar
7 Vsevolodov, N. N. Biomolecular Electronics. An Introduction Via PhotosensitiVe Proteins (Birkhauser: Boston, 1998) pp.125 Google Scholar
8 Nabiev, I. Sukhanova, A. Artemyev, M. Oleinikov, V. “Fluorescent colloidal particles as a detection tools in biotechnology systems”, Colloidal Nanoparticles in Biotechnology, ed. Elaissari, A. (Wiley & Sons Inc., London-Singapore-NY, 1998) pp.133–168.Google Scholar
9 Medintz, I. L. Trammell, S. A., Mattoussi, H. Mauro, J. M. J. Am. Chem. Soc. 126, 30–31 (2004).Google Scholar
10 Oesterhelt, D. Stoeckenius, W. Methods Enzymol. 311, 667 (1978).Google Scholar
11 Sukhanova, A. Devy, J. Venteo, L. Kaplan, H. Artemyev, M. Oleinikov, V. Klimov, D. Pluot, M. Cohen, J. H. M. Nabiev, I. Anal Biochem. 324, 60–67 (2004).Google Scholar