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Development of simplified biofilm sorption and diffusion experiment method using Bacillus sp. isolated from Horonobe Underground Research Laboratory

Published online by Cambridge University Press:  30 June 2014

Kotaro Ise ,
Tomofumi Sato ,
Yoshito Sasaki  and
Hideki Yoshikawa
Kotaro Ise
Affiliation:
Radionuclide Migration Research Group, Geological Isolation, Research and Development Directorate, Japan Atomic Energy Agency (JAEA), 4-33 Muramatsu Tokai-Mura, Naka-gun, Ibaraki 319-1194, Japan
Tomofumi Sato
Affiliation:
Radionuclide Migration Research Group, Geological Isolation, Research and Development Directorate, Japan Atomic Energy Agency (JAEA), 4-33 Muramatsu Tokai-Mura, Naka-gun, Ibaraki 319-1194, Japan
Yoshito Sasaki
Affiliation:
Radionuclide Migration Research Group, Geological Isolation, Research and Development Directorate, Japan Atomic Energy Agency (JAEA), 4-33 Muramatsu Tokai-Mura, Naka-gun, Ibaraki 319-1194, Japan
Hideki Yoshikawa
Affiliation:
Radionuclide Migration Research Group, Geological Isolation, Research and Development Directorate, Japan Atomic Energy Agency (JAEA), 4-33 Muramatsu Tokai-Mura, Naka-gun, Ibaraki 319-1194, Japan
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Abstract

We developed a simplified biofilm sorption and diffusion experiment method. The biofilms of the Bacillus cereus were incubated on cellulose acetate membrane filters (pore size 0.2 µm, diameter 47 mm) placed on thick NB broth agar medium (thickness was about 30 mm) to support sufficient biofilm growth of the Bacillus cereus. The thickness of the formed biofilms was about 1 mm. The formed biofilms were applied to through-diffusion method, which has been used to measure diffusion coefficient of crystalline and sedimentary rocks and clay minerals. The obtained copper sorption coefficient by batch experiments was about 100 ml/g (wet weight) at the case of the concentration of cupper ion was over 0.074mmol/L. And diffusion coefficients by through diffusion experiment was De=1.1 x 10-10 (m2/s). From these results, this simplified biofilm sorption and diffusion experiment may make possible to obtain these parameters with ease.

Keywords

diffusiongeologicbiological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1665: Symposium NW – Scientific Basis for Nuclear Waste Management XXXVII , 2014 , pp. 171 - 177
Copyright
Copyright © Materials Research Society 2014 

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References

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