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Assessment of marine biofilm attachment and growth for antifouling surfaces under static and controlled hydrodynamic conditions

Published online by Cambridge University Press:  19 July 2011

Maria Salta ,
Julian A. Wharton ,
Paul Stoodley ,
Robert J.K. Wood  and
Keith R. Stokes
Maria Salta
Affiliation:
national Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
Julian A. Wharton
Affiliation:
national Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
Paul Stoodley
Affiliation:
national Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
Robert J.K. Wood
Affiliation:
national Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
Keith R. Stokes
Affiliation:
national Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK. Physical Sciences Department, Dstl, Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK.
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Abstract

This investigation has assessed natural product antifouling performance of an isolated compound from a terrestrial source against marine biofilm forming bacteria, Cobetia marina and Marinobacter hydrocarbonoclasticus. Novel bioassay protocols using the hydrodynamic system and its well plate microfluidics capability were developed to test the in situ antifouling efficacy of the natural product against biofilm attachment under two shear stresses (0.07 and 0.3 Pa). The hydrodynamic results allowed for the first time the direct observation of the natural product influence on newly attached marine biofilms and the evolution of the antifouling affect with time. Biofilm attachment behaviour appeared to be markedly different in the presence of the natural product, illustrated by limited cluster and extracellular polymeric substance formation which suggests an interference of the bacterial attachment mechanisms. Ultimately, this is fundamental in developing greater understanding of the biofilm kinetics. These observations were confirmed using epifluoresence and confocal microscopy, with the additional corroborative data on bacterial cell integrity using the LIVE / DEAD nucleic acid kit.

Keywords

biologicalbiomimetic (chemical reaction)film
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1356: Symposium KK – Microbial Life on Surfaces—Biofilm-Material Interactions , 2011 , mrss11-1356-kk06-06
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

1. Salta, M., Wharton, J.A., Stoodley, P., Dennington, S., Goodes, L., Werwinski, S., Mart, U., Wood, R.J.K., Stokes, K.R., Special Issue on Green Tribology – Designing biomimetic antifouling surfaces, Phil Trans A Royal Society, 368, 4729–4754 (2010).10.1098/rsta.2010.0195Google Scholar
2. Maximilien, R., De Nys, R., Holmstrom, C., Gram, L., Kjelleberg, S., Steinberg, P., Bacterial fouling is regulated by secondary metabolites from the red alga Delisea pulchra, Aquat. Microbial. Ecol. 15, 233–246 (1998).10.3354/ame015233Google Scholar
3. Ekblad, T., Bergstrom, G., Ederth, T., Conlan, S., Mutton, R., Clare, A., Wang, S., Liu, Y., Zhao, Q., D’Souza, F., Donnelly, G., Willemsen, P., Pettitt, M., Callow, M., Callow, J., Liedberg, B., Poly (ethylene glycol)-containing hydrogel surfaces for antifouling applications in marine and freshwater environments, Biomacromolecules 9, 2775–2783 (2008).10.1021/bm800547mGoogle Scholar
4. Akesso, L., Pettitt, M., Callow, J., Callow, M., Stallard, J., Teer, D., Liu, C., Wang, S., Zhao, Q., Willemsen, P., The potential of nano-structured silicon oxide type coatings deposited by PACVD for control of aquatic biofouling. Biofouling, 25, 55–67 (2009).10.1080/08927010802444275Google Scholar
5. D’Souza, F., Bruin, A., Biersteker, R., Donnelly, G., Klijnstra, J., Rentrop, C., Willemsen, P., Bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials, J. Indust. Microbiol. Biotechnol. 37, 363–370 (2010).10.1007/s10295-009-0681-1Google Scholar
6. Benoit, M.R., Conant, C.G., Ionescu-Zanetti, C., Schwartz, M. and Matin, A., New Device for High-Throughput Viability Screening of Flow Biofilms, Appl. Environ. Microbiol. 76, 4136–4142 (2010).10.1128/AEM.03065-09Google Scholar
7. O’Brien, P., Molecular mechanisms of quinone cytocity, Chemico-Biological Interactions, 80, 1–41 (1991).10.1016/0009-2797(91)90029-7Google Scholar
8. Rieu, A., Briandet, R., Habimana, O., Garmyn, D., Guzzo, J., Piveteau, P., Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains, Appl. Environ. Microbiol. 74, 4491–4497 (2008).10.1128/AEM.00255-08Google Scholar
9. Beyenal, H., Lewandowski, Z., Internal and external mass transfer in biofilms grown at various flow velocities, Biotechnology Progress, 18, 55–61 (2002).10.1021/bp010129sGoogle Scholar