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Peptide Coated Titanium Rods Show Increased Bone Formation in a Rat Femur Model

Published online by Cambridge University Press:  15 February 2011

D.M. Ferris ,
G.D. Moodie ,
P.M. Dimond ,
M.G. Ehrlich  and
R.F. Valentini
D.M. Ferris
Affiliation:
MPPB Section, Brown University, Providence RI, 02912USA
G.D. Moodie
Affiliation:
MPPB Section, Brown University, Providence RI, 02912USA
P.M. Dimond
Affiliation:
Department of Orthopaedics, Rhode Island Hospital, Providence, RI 02912
M.G. Ehrlich
Affiliation:
Department of Orthopaedics, Rhode Island Hospital, Providence, RI 02912
R.F. Valentini
Affiliation:
MPPB Section, Brown University, Providence RI, 02912USA Department of Orthopaedics, Rhode Island Hospital, Providence, RI 02912
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Abstract

In vitro, groups have demonstrated that peptide modified surfaces influence short and long term cell responses like attachment, shape and function via cell receptors known as integrins. These receptors translate information to the nucleus via sets of complex signaling pathways. Little is known about the ability of these surfaces to influence the inherently complex in vivo environment, however. The present study was designed to evaluate the quality and quantity of new bone formed in response to gold coated titanium rods modified with the peptide sequence Arg-Gly-Asp-Cys (RGDC). Quantitative histomorphometric analysis of histologic sections perpendicular to the implant long axis showed a thicker (P < 0.01) shell of new bone formed in response to peptide modified implants (26.2 microns ± 1.9 vs. 20.5 microns ± 2.9) as early as 2 weeks. Mechanical pull-out testing conducted at 4 weeks revealed the average pull-out force of peptide modified rods was 38% greater than gold control rods. The significant difference in the thickness of new bone formed around the implant was maintained at 4 weeks (32.7 microns ± 4.6 vs. 22.6 microns ± 4.0). These results demonstrate the feasibility of developing peptide coated biomaterials designed to elicit a host response at the cell receptor level.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 550: Symposium GG/HH/II – Biomedical Materials-Drug Delivery, Implants and Tissue Engr , 1998 , 287
Copyright
Copyright © Materials Research Society 1999

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References

1. Berzins, A, Shah, B, Weinans, H and Sumner, D. J Biomed Mater Res. 1997, 34 (3) 337–340.Google Scholar
2. Boskey, A.L. (1989). Bone and Mineral, 6, 111–123.Google Scholar
3. Brandley, B and Schnaar, R. Anal Biochem. 1988, 172 (1) 270–278.Google Scholar
4. Branemark, R, Ohrnell, L, Nilsson, P and Thomsen, P. Biomaterials. 1997, 18 (14) 969–978.Google Scholar
5. Branemark, R, Ohrnell, L, Skalak, R, Carlsson, L and Branemark, P. J Orthop Res. 1998, 16 (1) 61–69.Google Scholar
6. Chae, JC, Collier, JP, Mayor, MB, Surprenant, VA and Dauphinais, LA. J Biomed Mater Res. 1992, 26 93–102.Google Scholar
7. Chen, C, Mrksich, M, Huang, S, Whitesides, G and Ingber, D. Science. 1997, 276 (5317) 1425–1428.Google Scholar
8. Davies, J. E. (1996). The Anatomical Record, 245, 426–445.Google Scholar
9. Dee, K, Rueger, D, Andersen, T and Bizios, R. Biomaterials. 1996, 17 (2) 209–215.Google Scholar
10. Frank, JD, Balena, R, Masarachia, P, Seedor, JG and Cartwright, ME. Histochemistry. 1993, 99 295–301.Google Scholar
11. Friedman, RI, Bauer, TW, Garg, K, Jiang, M, Yuehuei, H and Draughn, RA. Journal of Applied Biomaterials, 1995, 6 231–235.Google Scholar
12. Hayashi, K, Matsuguchi, N, Uenoyama, K, Kanemaru, T and Sugioka, Y. J Biomed Mater Res. 1989, 23 1247–1259.Google Scholar
13. Margel, S, Vogler, E, Firment, L, Watt, T, Haynie, S and Sogah, D. J Biomed Mater Res. 1993, 27 (12) 1463–1476.Google Scholar
14. Massia, S and Hubbell, J. Anal Biochem. 1990, 187 (2) 292–301.Google Scholar
15. Marks, A. and Popoff, S. (1988). The American Journal of Anatomy, 183, 1–44.Google Scholar
16. Moodie, GD, Sherling, MA, Morgan, H, Ferris, DM, Chen, CY, Valentini, RF. 24th Annual Meeting of the Society for Biomaterials, April 22-26, 1998, San Diego, CA, USA p. 116 Google Scholar
17. Mrksich, M, Chen, C., Xia, Y, Dike, L, Ingbe, rD and Whitesides, G. Proc Natl Acad Sci U S A. 1996, 93 (20) 10775–10778.Google Scholar
18. Mrksich, M and Whitesides, G. Annu Rev Biophys Biomol Struct. 1996, 25 55–78.Google Scholar
19. Mrksich, M, Dike, L, Tien, J, Ingber, D and Whitesides, G. Exp Cell Res. 1997, 235 (2) 305–313.Google Scholar
20. Muller, R and Patsalis, T. Arch Orthop Trauma Surg. 1997, 116 (6-7) 334–337.Google Scholar
21. Neff, J, Caldwell, K and Tresco, P. J Biomed Mater Res. 1998, 40 (4) 511–519.Google Scholar
22. Nimb, L, Jensen, JS and Gotfredsen, K. Journal of Biomedical Materials Research. 1995, 29 1477–1482.Google Scholar
23. Prime, K and Whitesides, G. Science. 1991, 252 (5010) 1164–1167.Google Scholar
24. Rashmir-Raven, AM, Richardson, DC, Aberman, HM and DeYoung, DJ. Journal of Applied Biomaterials. 1995, 6 237–242.Google Scholar
25. Reddi, A. (1997) Cell, 89, 159–161.Google Scholar
26. Rezania, A, Thomas, C., Branger, A, Waters, C and Healy, K. J Biomed Mater Res. 1997, 37 (1) 9–19.Google Scholar
27. Robey, P. G. (1989). Endocrinology and Metabolism Clinics of North America, 18(4), 859–901.Google Scholar
28. Silin, V, Weetall, H and Vanderah, D. J Colloid Interface Sci. 1997, 185 (1) 94–103.Google Scholar
29. Singhvi, R, Kumar, A, Lopez, G et al. Science. 1994, 264 (5159) 696–698.Google Scholar
30. Stanford, C. and Keller, J. (1991). Critical Reviews in Oral Biology and Medicine, 2(1), 83–101.Google Scholar
31. Tisdel, CL, Goldberg, VM, Parr, JA, Bensusan, JS, Staikoff, LS and Stevenson, S. The Journal of Bone and Joint Surgery, American Volume. 1994, 76–A (2) 159–171.Google Scholar
32. Valentini, R.F., Ferris, D., DiGiovanni, C., Dimond, P., Sherling, M., Moodie, G., Crisco, T., Labrador, D., Ehrlich, M. presented at The 23rd Annual Meeting of the Society for Biomaterials, April 30 May 4, 1997, New Orleans, Louisiana, USA. Google Scholar
33. Wink, T, van Zuilen, S, Bult, A and van Bennkom, W. Analyst. 1997, 122 (4) 43R–50R.Google Scholar
34. Wong, M, Eulenberger, J, Schenk, R and Hunziker, E. J Biomed Mater Res. 1995, 29 1567–1575.Google Scholar
35. Xiao, Y and Truskey, GA. Biophys J 1996, 71 (5) 2869–2884.Google Scholar
36. Zou, L, Moodie, GD, Sherling, MA, Keeping, HS, Ehrlich, MG, Valentini, RF. 24th Annual Meeting of the Society for Biomaterials, April 22-26, 1998, San Diego, CA, USA p. 161.Google Scholar