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Effects of Ionic Flow and Amelogenins on the Lengthwise Growth of Octacalcium Phosphate Crystals in a Model System of Tooth Enamel Formation

Published online by Cambridge University Press:  01 February 2011

M. Iijima ,
Y. Moriwaki ,
H.B. Wen ,
T. Takagi ,
A.G. Fincham  and
J. Moradian-Oldak
M. Iijima
Affiliation:
Asahi University School of Dentistry, Dental Materials and Technology, 1851-1 Hozumi, Hozumi-cho, Motosugun, Gifu 501-0296, Japan.
Y. Moriwaki
Affiliation:
Asahi University School of Dentistry, Dental Materials and Technology, 1851-1 Hozumi, Hozumi-cho, Motosugun, Gifu 501-0296, Japan.
H.B. Wen
Affiliation:
University of Southern California, School of Dentistry, Center for Craniofacial Molecular Biology, USA.
T. Takagi
Affiliation:
Tokyo Medical and Dental University, School of Dentistry, Oral Biology, Japan.
A.G. Fincham
Affiliation:
University of Southern California, School of Dentistry, Center for Craniofacial Molecular Biology, USA.
J. Moradian-Oldak
Affiliation:
University of Southern California, School of Dentistry, Center for Craniofacial Molecular Biology, USA.
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Abstract

This paper briefly reviews our recent studies, which aimed to investigate the effects of 1) the Ca2+ and PO43- ions flow and 2) amelogenins on the lengthwise growth of octacalcium phosphate (OCP), which is a potent precursor of enamel apatite crystal. OCP crystals were grown at 37°C in a dual membrane system under various amount of ionic inflow into a reaction space, using 1) 5-30mM Ca and PO4 solutions as ionic sources and 2) extracted bovine amelogenin and recombinant murine amelogenins (rM179, rM166). With an increase in the amount of Ca2+ and/or PO43- ions flow, the length of OCP crystal increased, while the width decreased. As a result, the length to width (L/W) ratio of crystal changed from 3 to 95, while the width to thickness (W/T) ratio from 32 to 9. The effect of amelogenins was unique, regardless of the type of amelogenins: Rod-like and prism-like OCP crystals with large L/W (61∼107) and small W/T (1.3∼2.2) ratios were formed in 10% amelogenin gels. In contrast, characteristic ribbon-like OCP crystals grew without protein and with gelatin, albumin, polyacrylamide gel and agarose gel. Specific interaction of amelogenins with OCP crystal was ascribed to the self-assembly property of amelogenin molecules and their hydrophobic nature. It was suggested that ionic flow and amelogenins play some critical roles in the elongated growth of enamel crystals.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N6.4
Copyright
Copyright © Materials Research Society 2002

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