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Watching Rafts Move Within Cells: A Fluorescence Microscope-Based Transport Assay
Published online by Cambridge University Press: 14 March 2018
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Imagine a raft in a canal between point A and point B. On that raft is a visible (fluorescent) cargo. Also, attached to that raft is a motor that will propel the raft only from A to B (anterograde transport). When the raft gets to point B, another motor is attached that can propel the raft, and its cargo, and the anterograde motor, back to point A (retrograde transport). Within a cell, the canals are microtubLiles, and a lot is known about anterograde and retrograde transport in some systems, but these phenomena have not been directly observed in a living, intact animal. Until now, that is. In a pair of very interesting papers, the laboratory of Jonathan Scholey has shown us convincing micrographs of anterograde and retrograde transport in an important animal model.
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- Research Article
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- Copyright © Microscopy Society of America 2000
References
Orozco, J.T., Wedaman, K.P., Signor, D., Brown, H., Rose, L., and Scholey, J.M., Movement of motor and cargo along cilia, Nature
398:674, 1999.CrossRefGoogle ScholarPubMed
Signor, D., Wedaman, K.P., Orozco, J.T., Dwyer, N.D., Bargmann, C.I., Rose, L. S., and Scholey, J.M., Role of class DHC1b dynein in retrograde transport of IFT motors and [FT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans, J. Cell Biol.
147:519-530, 1999. Video images from this study can be accessed at http://www.mcb.ucdavis.edu/faculty-labs/scholey/CrossRefGoogle Scholar
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