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Visualizing Gene Expression in Real-Time

Published online by Cambridge University Press:  14 March 2018

Stephen W. Carmichael*
Affiliation:
Mayo Clinic

Extract

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Gene expression has been visualized for a few decades, but in static forms such as blots and gene chips. Susan Janicki, Toshiro Tsukamoto, Sim one Salghetti, William Tansey, Ravi Sachidanandam, Kannanganattu Prasanth, Thomas Ried, Yaron Shav-Tal, Edouard Bertrand, Robert Singer, and David Spector have recently designed a cell line in which gene expression can be observed with stunningly accurate spatial and temporal resolution. Gene expression is a cascade of events beginning with transcription of RNA from the DNA template and ending with translation into a protein sequence. Janicki et al. were able to visualize the entire process at the levels of DNA, RNA, and proteins in living cells!

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2005

Footnotes

1

The author gratefully acknowledges Dr. David Spector for reviewing this article.

References

Janicki, S.M., Tsukamoto, T., Salghetti, S.E., Tansey, W.P., Sachidanandam, R., Prasanth, K.V., Ried, T., Sharon Shav-Tal, Y., Bertrand, E., Singer, R.H., and Spector, D.L., From silencing to gene expression: Real-time analysis in single genes, Cell 116:683-698, 2004.CrossRefGoogle Scholar