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VisBio: a Flexible Open-Source Visualization Package for Multidimensional Image Data

Published online by Cambridge University Press:  14 March 2018

Curtis T. Rueden*
Affiliation:
Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison
Kevin W. Eliceiri
Affiliation:
Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison

Extract

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Over the past few years there has been a dramatic improvement in microscopy acquisition techniques, in effective imaging modalities as well as raw hardware performance. As the microscopist's available tools become more sophisticated and diverse—e.g., time-lapse, Z sectioning, multispectra, lifetime, nth harmonic, polarization, and many combinations thereof—we face a corresponding increase in complexity in the software for understanding and interpreting the resultant data. With lifetime imaging, for example, it is overwhelming to study the raw numbers; instead, an exponential curve-fitting algorithm must be applied to extract meaningful lifetime values from the mass of photon counts recorded by the instrument.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2006

References

1. Eliceiri, K. W. and Rueden, C. (2005) Tools for Visualizing Multidimensional Images from Living Specimens. Photochemistry and Photobiology 81(5), 11161122.Google Scholar
2. Abramoff, M. D., Magalhaes, P. J. and Sam, S. J. (2004) Image processing with Image J. Biophot. Int. 3642.Google Scholar
3. Swedlow, J. R., Goldberg, I., Brauner, E. and Sorger, R K. (2003) Informatics and quantitative analysis in biological imaging. Science 300(5616), 100102.CrossRefGoogle ScholarPubMed
4. Rueden, C, Eliceiri, K. W. and White, J. G. (2004) VisBio: a computational tool for visualization of multidimensional biological image data. Traffic 5, 17.Google Scholar
5. Hibbard, W., Rueden, C., Emmerson, S., Rink, T., Glowacki, D., Whittaker, T., Murray, D., Fulker, D. and Anderson, J. (2005) Java distributed components for numerical visualization in VisAD. Communications of the ACM 48(3), 98104.Google Scholar