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Vascular Corrosion Casting Can Provide Quantitative as Well as Morphological Information on the Microvasculature of Organs and Tissues

Published online by Cambridge University Press:  14 March 2018

Fred E. Hossler
Fred E. Hossler*
Affiliation:
J.H. Guillen College of Medicine, East Tennessee State University

Extract

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Complete casts of the vasculature of organs and tissues are obtained by infusing low viscosity resins into the vasculature and allowing the resin to polymerize. Dissolving away the surrounding tissue with alkali leaves a model of the intricate, three-dimensional distribution of vessels in that tissue, which is not easily obtainable by any other means, and which can then be studied with scanning electron microscopy (SEM), Because well prepared casts appear to faithfully replicate the true vascular anatomy of organs including the dimensions of vessels and details of imprints of the endothelial cells lining their lumens, they must also contain quantitative information about that vasculature.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 7 , September 1998 , pp. 14 - 15
Copyright
Copyright © Microscopy Society of America 1998

References

Literature Cited

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2. Hossler, F.E., Douglas, J.E., and Douglas, L.E. 1986 Anatomy and morphometry of myocardial capillaries studied with vascular corrosion casting and scanning electron microscopy; a method for rat heart. Scanning Electron Microscopy/1986/IV: 1469-1475.Google Scholar
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