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Trapping Subfemtoliter-Sized Vesicles, and Analyzing Them

Published online by Cambridge University Press:  14 March 2018

Stephen W. Carmichael
Stephen W. Carmichael*
Affiliation:
Mayo Clinic

Extract

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The chemical analysis of secretory vesicles has long been limited by the fact that many vesicles are analyzed together, resulting in an averaging of a population. Differences between vesicles within that population could not be detected. Wouldn't it be great if vesicles could be analyzed one at a time? You guessed it, this breakthrough has been recently achieved.

The technique of capillary electrophoresis (CE) was modified by Daniel Chiu, Sheri Lillard, Richard Scheller, Richard Zare, Sandra Rodriguez-Cruz, Evan Williams, Owe Orwar, Mats Sandberg, and Anders Lundqvist so that vesicles could be probed singly.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 4 , May 1998 , pp. 3 - 4
Copyright
Copyright © Microscopy Society of America 1998

References

2 Chiu, D.T., Lillard, S.J., Scheller, R.H., Zare, R.N., Rodriguez-Crnz, S.E., Williams, E.R., Onvar, W., Sandberg, M.. and Lundqvist, J.A.. Probing single secretory vesicles with capillary electrophoresis. Science 279: 1190–1193. 1998 CrossRefGoogle ScholarPubMed