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Tissue Digestion for Ferruginous Bodies for Light Microscopy

Published online by Cambridge University Press:  14 March 2018

Margaret Gondo
Margaret Gondo*
Affiliation:
Baylor College of Medicine

Extract

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Asbestos fibers are commonly called ferruginous bodies due to the iron content of the fibers. After they are inhaled, the asbestos fibers become coated with glycoprotein and hemosiderin, deposited by macrophages.

This procedure may be used on either formalin fixed, paraffin embedded blocks or formalin fixed wet tissue. Because this procedure will result in complete destruction of the tissue sample used, it is very important to obtain written approval documenting this fact from the individual requesting this procedure, if the procedure is being done for someone else. The tissue should be from the lung parenchyma. Also, it is a good idea to use sterile, disposable tubes throughout this procedure to limit the possibility of contamination.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 1 , January 1998 , pp. 8 - 11
Copyright
Copyright © Microscopy Society of America 1998

References

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