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TEM Morphometry Reveals Membrane Deficits in Parietal Cells Lacking Specific Ion Transporters

Published online by Cambridge University Press:  14 March 2018

ML Miller*
Affiliation:
University of Cincinnati, College of Medicine
LR Gawenis
Affiliation:
University of Cincinnati, College of Medicine
A Andringa
Affiliation:
University of Cincinnati, College of Medicine
GE Shull
Affiliation:
University of Cincinnati, College of Medicine

Extract

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The late 1940s marked the appearance of the first publications using transmission electron microscopy (TEM) and by 1950, with improved embedding and sectioning methodology, reports began to include mammalian tissues (PubMed). However, 15 years lapsed before morphometry and stereology were integrated into TEM studies (Loud 1965; Elias 1971; Weibel 1969). The utility of a quantitative approach to electron microscopy was obvious, but its popularity has waxed and waned considerably over the last half century; its staunch supporters, however, have stayed the course (Weibel 2001; Diaspro 2004). Early computers, though slow and unreliable, brought some degree of automation to morphometry in the 1980s, but did little to ease tedium or increase accuracy (Caruntu 2002).

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2005

References

Bell, SM, Schreiner, CM, Schultheis, PJ, Miller, ML, Evans, RL, Vorhees, CV, Shull, GE, Scott, WJ. Targeted disruption of the murine Nhe1 locus induces ataxia, growth retardation, and seizures. Am J Physiol 1999, 276(4 Pt 1):C788-95.CrossRefGoogle Scholar
Caruntu, ID. Highly effective techniques in computerized dental tissue morphometry. J Cell Mol Med 2002, 6(4):631–42. Diaspro, A. Image processing in optical microscopy. Micros Res Tech 2004, 64:87-88.Google ScholarPubMed
Elias, H, Hennig, A, Schwartz, DE. Stereology: applications to biomedical research. Physiol Rev 1971, 51(1):158200.Google Scholar
Gawenis, LR, Hut, H, Bot, AG, Shull, GE, de Jonge, HR, Stien, X, Miller, ML, Clarke, LL. Electroneutral sodium absorption and electrogenic anion secretion across murine small intestine are regulated in parallel. Am J Physiol Gastrointest Liver Physiol. 2004a Dec; 287(6):G1 140-9. Epub 2004b Jul 29.Google Scholar
Gawenis, LR, Ledoussal, C, Judd, LM, Prasad, V, Alper, SL, Stuart-Tilley, A, Woo, AL, Grisham, C, Sanford, LP, Doetschman, T, Miller, ML, Shull, GE. Mice with a targeted disruption of the AE2 Cl/HCO3 exchanger are achlorhydric. J Biol Chem 2004b, 279(29):30531 -9. Epub 2004a.Google Scholar
Gil, J, Wu, H, Wang, BY. Image analysis and morphometry in the diagnosis of breast cancer. Microsc Res Tech 2002, 59(2):109-18. Loud, AV, Barney, WC, Pack, BA. Quantitative evaluation of cytoplasmic structures in electron micrographs. Lab Invest 1965, 14:996-1008.Google Scholar
Miller, ML, Judd, LM, Van Driel, IR, Andringa, A, Flagella, M, Bell, SM, Schultheis, PJ, Spicer, Z, Shull, GE. The unique ultrastructure of secretory membranes in gastric parietal cells depends upon the presence of H+,K+-ATPase. Cell Tissue Res 2002, 309(3):369-80.Google ScholarPubMed
Okunade, GW, Miller, ML, Pyne, GJ, Sutliff, RL, O'Connor, KT, Neumann, JC, Andringa, A, Miller, DA, Prasad, V, Doetschman, T, Paul, RJ, Shull, GE. Targeted ablation of plasma membrane Ca2+ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA 1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem 2004, 279(32):33742-50.Google Scholar
Schultheis, PJ, Clarke, LL, Meneton, P, Harline, M, Boivin, GP, Stemmermann, G, Duffy, JJ, Doetschman, T, Miller, ML, Shull, GE. Targeted disruption of the murine Na+/H+ exchanger isoform 2 gene causes reduced viability of gastric parietal cells and loss of net acid secretion. J Clin Invest 1998a, 101(6):1243- 53.Google Scholar
Schultheis, PJ, Clarke, LL, Meneton, P, Miller, ML, Soleimani, M, Gawenis, LR, Riddle, TM, Duffy, JJ, Doetschman, T, Wang, T, Giebisch, G, Aronson, PS, Lorenz, JN, Shull, GE. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nat Genet 1998, 9(3):282-5.Google Scholar
Shull, GE, Miller, ML, Schultheis, PJ. Lessons from genetically engineered animal models VIII. Absorption and secretion of ions in the gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 2000, 278(2):G185-90.Google ScholarPubMed
Spicer, Z, Miller, ML, Andringa, A, Riddle, TM, Duffy, JJ, Doetschman, T, Shull, GE. Stomachs of mice lacking the gastric H,K-ATPase alpha-subunit have achlorhydria, abnormal parietal cells, and ciliated metaplasia. J Biol Chem 2000, 275(28):21555-65.Google ScholarPubMed
Weibel, ER. Why measure lung structure? Am J Respir Crit Care Med. 2001, 163(2):314-5. Weibel ER. Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 1969, 26:235-302.Google Scholar