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TEM Flat Embedding Technique

Published online by Cambridge University Press:  14 March 2018

Louisa Howard
Louisa Howard*
Affiliation:
Dartmouth College, Duane Compton & Alejandro Saredi, Dartmouth Medical School

Extract

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Individual BHK-21 (Baby Hamster Kidney-21} cells growing on 25 mm photo-etched alpha-numeric glass coverslips (Belico Co.) are identified by phase contrast microscopy and photographed. (Saredi, Howard and Compton, 1995), This method should work for other cell lines, but likely will require some empirical adjustment of the times, concentrations, and pH.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 7 , September 1997 , pp. 24 - 25
Copyright
Copyright © Microscopy Society of America 1997

References

Moore, M.J., 1975, Removal of glass coverslips from cultures flat embedded in epoxy resins using HF. Microscopy 104, 205.CrossRefGoogle Scholar
Conly, Rieder and Samuel, Bowser, 1987, “Correlative LM and EM on the same epoxy section”, in Correlative Microscopy in Biology. Chapter 11 (en. M.A Hayet), pp. 249–277, Academic Press Google Scholar
Saredi, A., Howard, L. and Compton, D., 1996 “NuMa assembles into an extensive fliamentous structure when expressed in the cell cytoplasm”. J. Cell Sci. 109: 619–630.Google Scholar