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Special Topic: Advanced Basics of Immunostaining and Antigen Retrieval

Published online by Cambridge University Press:  14 March 2018

W. Gray (Jay) Jerome
W. Gray (Jay) Jerome*
Affiliation:
Vanderbilt University Medical Center
*
[email protected]

Extract

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The ability to immunologically link microscopic tags to specific proteins has produced major advances in all forms of microscopy. Initially researchers needed to make and label their own antibodies. This required a good working knowledge of immunology. Today's labeled antibodies are readily available from a variety of vendors. Although this has greatly facilitated the expansion of immunolabeling techniques, it has also led to their use by researchers with little or no background in immunology.

Type
Research Article
Information
Microscopy Today , Volume 11 , Issue 4 , August 2003 , pp. 26 - 29
Copyright
Copyright © Microscopy Society of America 2003

References

Some Useful References

Polak, JM, van Noorden, S. 1997. Introduction to Immunocytochemistry. Second Edition. New York, Springer-Veriag.- A nice basic, easy to read, introduction with useful protocols. Google Scholar
Javois, LC (editor). 1999. Immunocytochemical Methods and Protocols. Second Edition. Totowa NJ, Humana Press- Detailed description of many important aspects of Immunocytochemistiy with detailed protocols. Google Scholar
Shi, S-R, Gu, J, Taylor, CR (Eds). 2000. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. Westboraugh MA, Bio Techniques Press. - Historical review and authoritative protocols. Google Scholar
Shi, S-R, Cote, RJ, Taylor CR, 2001. Antigen retrieval techniques: current perspectives. J Histochem Cytochem 49:931-937, - Brief review of key variables. Google Scholar
Hayat, MA. 2002. Microscopy, Immunohistochemistry, and Antigen Retrieval Methods for Light and Electron Microscopy, New York, Kluwer Academic / Plenum Publishers. - Good review, particularly concerning electron microscopy antigen retrieval. Google Scholar