Adobe Photoshop may not come to mind as a program that is useful for segmenting (separating regions of interest from other visual information), but, in many ways, its functions can help scientists gain several end results. These include the ability to fully or partially automate the segmentation of entire directories of image files, the ability to better separate regions of interest from background, and the ability to narrow histograms (making the selection of a grayscale value less subjective when setting a threshold value).