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Reconstructing What Was: Software Applied To Serial Section TEM

Published online by Cambridge University Press:  14 March 2018

Marcia D. Feinberg  and
John C. Fiala
Marcia D. Feinberg*
Affiliation:
Boston University, Boston MA
John C. Fiala
Affiliation:
Boston University, Boston MA
*
[email protected]

Extract

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The well-known recipe for serial reconstruction in microscopy consists of the following six steps: fix and embed specimen, cut and mount sections for microscopy, image sections, align images in the computer, identify and trace profiles in images, generate three-dimensional (3D) visualizations. The free software program Reconstruct facilitates the final steps of this process for many types of microscopy, including scanning electron or ion beam microscopy applied to material and light microscopy applied to biological samples. We describe here how serial sectioning and transmission electron microscopy (TEM) can be combined with the Reconstruct software to generate large volumes of soft tissue ultrastructure for analysis.

Type
Research Article
Information
Microscopy Today , Volume 15 , Issue 5 , September 2007 , pp. 26 - 31
Copyright
Copyright © Microscopy Society of America 2007

References

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