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Preparation of Protein a Gold

Published online by Cambridge University Press:  14 March 2018

Paul Webster
Paul Webster*
Affiliation:
Center for Cell Imaging, Yale School of Medicine

Extract

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Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years, colloidal gold particles have become a useful tool in microscopy for staining tissues and sections. Colloidal gold particles are especially useful for biological electron microscopy, Some of the reasons why are listed below.

  1. * Homogeneous preparations of particles varying in size from 3μm to 20μm can be easily prepared.

  2. * Colloidal gold suspensions are inexpensive to prepare. Most proteins can be easily coupled to colloidal gold particles.

  3. * Most proteins can be easily coupled to colloidal gold particles.

  4. * Proteins coupled to gold particles do not appear to lose their biological activity.

  5. * The colloidal gold particles can be easily seen in the electron microscope.

  6. * Colloidal gold does not naturally occur in biological material. Therefore, if you see it, it is because you put it there.

  7. * Colloidal gold probes can be used for light microscopy, The larger gold particles can be directly observed by the light microscope. Small particles are detected by silver enhancement or epipolarized illumination.

  8. * The same probes can be used for both LM and TEM imrnunocytochemistry.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 5 , June 1997 , pp. 12 - 13
Copyright
Copyright © Microscopy Society of America 1997

References

Slot, J.W. & Geuze, H.J, 1981. Sizing of protein A-colliodal gold probes for immunoelectron microscopy. J .Cell Biol. 90,533-536.Google Scholar
Slot, J.W. & Geuze, H.J, 1981. A new method of preparing gold probes for multiple-labeling cytochemistry. Europ. J.Cell Biol, 38, 87-93.Google Scholar