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Pioloform F Instead of Formvar as a Support Film for TEM

Published online by Cambridge University Press:  14 March 2018

Victoria J. Madden
Victoria J. Madden*
Affiliation:
University of North Carolina – Chapel Hill

Extract

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Pioloform F (Wacker Chemie Co., Munich, Germany) is an excellent plastic for use as a support film for electron microscopy. The advantages of Pioloform F are high mechanical and thermal stability, and low material bulk (Stockem, 1970). Because of these properties, thinner films than Formvar or collodion may be used and remain stable under the electron beam without subsequent stabilization by carton coating. Thin support films of gray interference color are prepared by dissolving 0.5 grams of Pioloform F powder in 100 milliliters of anhydrous chloroform (Stockem, 1970), using the dip method described by Reimer (1967).

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 10 , December 1998 , pp. 22 - 23
Copyright
Copyright © Microscopy Society of America 1998

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