No CrossRef data available.
Article contents
Osmium as an Immunostain Enhancement
Published online by Cambridge University Press: 14 March 2018
Extract
Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Since we do our paraffin-section DAB immunostaining in an EM lab, we often use osmium to darken the reaction product. We simply put a drop of our standard 1% cacodylate-buffered osmium tetroxide on each section in a hood and rinse it off in running tap water after about 2 seconds. It makes light DAB reaction product pretty dark brown, and adds a light brownish tint to the rest of the section. The reaction increases the contrast quite a bit.
- Type
- Microscopy 101
- Information
- Copyright
- Copyright © Microscopy Society of America 1999
You have
Access