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Osmium as an Immunostain Enhancement

Published online by Cambridge University Press:  14 March 2018

Hal Hawkins*
Affiliation:
University of Texas Medical Branch

Extract

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Since we do our paraffin-section DAB immunostaining in an EM lab, we often use osmium to darken the reaction product. We simply put a drop of our standard 1% cacodylate-buffered osmium tetroxide on each section in a hood and rinse it off in running tap water after about 2 seconds. It makes light DAB reaction product pretty dark brown, and adds a light brownish tint to the rest of the section. The reaction increases the contrast quite a bit.

Type
Microscopy 101
Copyright
Copyright © Microscopy Society of America 1999