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A New Universal Acrylic Embedding Resin for Both Light and Electron Microcopy

Published online by Cambridge University Press:  14 March 2018

Donald P. Cox
Donald P. Cox*
Affiliation:
Goldmark Biologicals
*
Tel.: (908)859-2631 - Fax: (908)859-2875

Extract

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Successful immunolabeling in electron microscopy of animal and plant tissues requires a combination of excellent antigen preservation while maintaining the original structure of the tissue. One important element is tissue embedding which accomplishes two goals for the immunohistochemist, the preservation of tissue specimen structure and maintenance of biological antigenicity. Tissue embedding in plastic resins is a common method in which several important elements must be considered.

  1. 1. Fine tissue structure must not be damaged by the polymerization.

  2. 2. The plastic must be stable to the electron beam.

  3. 3. Light scattering properties of the plastic should be minimal.

  4. 4. The plastic should cut easily.

  5. 5. The plastic must be of sufficiently low viscosity to infiltrate the tissue.

Type
Research Article
Information
Microscopy Today , Volume 2 , Issue 4 , July 1994 , pp. 21 - 22
Copyright
Copyright © Microscopy Society of America 1994

References

1. Dawes, CJ (1984) Biological Techniques for Transmission & Scanning Electron Microscopy. Ladd Research Industries, Burlington, VT Google Scholar
2. Causton, BE (1984) The choice of resins for electron immunocytochemistry, In (Polak, ; Varndell, eds.) Immunolabeling for Electron Microscopy. Elvsevier Science Publishers, New York, pp 29–36.Google Scholar
3. Product discovered and developed by Dr. C.S. Scala, University of BolognaGoogle Scholar
4. Scala, C.S. et al (1992) J Histochem Cytochem 40, 1799–1804 Google Scholar
5. Scala, CS et al (1993) Histochem J 25, 670-7Google Scholar
6. Manara, GC et al (1993) Eur J Dermatol 3, 235-3.Google Scholar