Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-08T07:53:44.793Z Has data issue: false hasContentIssue false
  • English
  • Français

Mounting Media and Antifade Reagents

Published online by Cambridge University Press:  14 March 2018

Tony J. Collins
Tony J. Collins*
Affiliation:
Wright Cell Imaging Facility, Toronto Western Research Institute
*
[email protected]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In the biomedical sciences, samples are mounted in a wide variety of media for examination by microscope. There are a wide variety of mounting media available with a correspondingly wide range of properties. Using the incorrect mounting medium may cause signal loss and optical aberrations; the correct mounting medium avoids such aberrations and preserves fluorescence signal with “anti-fading” properties. This article introduces mounting media for fluorescence microscopy, providing descriptions of their constituents and their properties, as well as accounts of users' experience

More detailed reviews of antifade reagents have been published by Ono et al. and Longin et al.. Papers describing the effect of refractive index (RI) mismatch have been published by Diaspro et al. and Hell et al..

Type
Research Article
Information
Microscopy Today , Volume 14 , Issue 1 , January 2006 , pp. 34 - 39
Copyright
Copyright © Microscopy Society of America 2006

References

Reference List

(1) Ono, et al. J Histochem Cytochem 2001; 49(3):305–312.Google Scholar
(2) Longin, et al. J Histochem Cytochem 1993; 41(12):1833–1840.CrossRefGoogle Scholar
(3) Diaspro, et al. Appl Opt 2002; 41(4):685–690.Google Scholar
(4) Hell, et al. Journal of Microscopy 1993; 169(3):391–105.Google Scholar
(5) Bacallao, et al. In: Pawley, JB, editor. Handbook of confocal icroscopy. New York: Plenum Press, 1995: p311–325.Google Scholar
(6) Johnson, et al. Journal of Immunological Methods 1982; 55(2):231–242.Google Scholar
(7) Song, L et al. Biophys J 1995; 68(6):2588–2600.CrossRefGoogle Scholar
(8) Paz-Miguel, et al. J Immunol 1999; 163(10):5399–5410.Google Scholar
(9) Steglich, et al. FEBS Letters 2003; 553(l-2):79–84.Google Scholar