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Influence of Illumination Conditions on Temperature in Sample Cell and the Output of a Quadrant Detector in an Optical Tweezers System

Published online by Cambridge University Press:  14 March 2018

Yuqiang Jiang ,
Honglian Guo ,
Chunxiang Liu ,
Zhaolin Li ,
Bingying Cheng ,
Daozhong Zhang  and
Suotang Jia
Yuqiang Jiang*
Affiliation:
College of Physics and Electronics Engineering, Shanxi University, TaiyuanP. R. China Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China Shanxi Datong University, DatongP. R. China
Honglian Guo
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Chunxiang Liu
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Zhaolin Li
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Bingying Cheng
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Daozhong Zhang
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Suotang Jia
Affiliation:
College of Physics and Electronics Engineering, Shanxi University, TaiyuanP. R. China
*
[email protected]

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In an optical tweezers system, the output signal of a photodiode quadrant detector and the temperature in a sample cell are two key factors for the quantitative measurements of mechanical properties of living biological objects such as cells, organelles and macro-molecules. In order to enhance the output of a quadrant detector and effectively control the temperature in a sample cell, the dependence of the temperature in the sample cell and the output of the quadrant detector for different illumination conditions are studied. The results show that appropriate illumination conditions can ensure both nearly constant temperatures in the cell and the desired output signal, which provides for the possibility of highprecision and damage-free analysis of living biological objects.

Type
Research Article
Information
Microscopy Today , Volume 13 , Issue 3 , May 2005 , pp. 44 - 49
Copyright
Copyright © Microscopy Society of America 2005

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