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Image Stitching Using Photoshop

Published online by Cambridge University Press:  14 March 2018

Jerry Sedgewick*
Affiliation:
University of Minnesota

Extract

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Several images can be stitched together (sometimes referred to as “montaging”) through the use of Photoshop. Edges of the images can be feathered and the process automated to reduce the hours of work that can go into this procedure.

Before going any further, let me be quick to say that more than one company has developed protocols to accomplish image stitching. When your lab is looking at large fields, while needing to resolve fine details, then I would strongly recommend the purchase of a system designed for this purpose. Issues surrounding motorized stage accuracy, problems with tilting when a camera is perched on a long optical tube, and image to image correction for contrast, color and uneven illumination can all contribute to confounding issues when image stitching. These companies have worked through these problems with off-the-shelf systems.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2004