In a recent microscopy discussion group, a correspondent questioned the use of a contrast enhancement method applied to a microscopy image for publication. What kinds of image processing, folk asked, are “acceptable” in a general sense? What kinds of image processing should be noted in the text or caption and what kind of images should be archived?

It is an interesting set of questions, and I face them wearing three hats, I am a forensic pathologist who performs forensic image interpretation in the investigation of homicide and assault, an anatomic pathologist with an interest in confocal microscopy, and a computer scientist with training in image processing and computer vision. Image processing is part and parcel of my everyday work. I use tools such as contrast enhancement, debarring, and photogrammetry for image interpretation. I build tools for visualization in my confocal work. I collaborate in the design of data acquisition devices for the evaluation of crime scenes and bodily injury.