Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T11:04:39.994Z Has data issue: false hasContentIssue false

HMDS and Specimen Drying for SEM:

Published online by Cambridge University Press:  14 March 2018

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Hexamethyltiisilizane (HMDS) is an excellent method of chemical drying of hydrated specimens. There are several variables involved in its use, the most easily controlled being the number of transitional steps from 100% eihanol (EtOH) to 100% HMDS and the drying temperature.

Fixation and dehydration are the same for both HMDS and CPD. Once thespecimenisinthefinal 100% ethanol, it must then be transferred to 100% HMDS through a graded series of ethanol-HMDS mixtures.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 1997