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Endogenous Fluorescence Identifies Dead Cells In Plants

Published online by Cambridge University Press:  14 March 2018

Jan S. Ryerse ,
Paul C. C. Feng  and
R. Douglas Sammons
Jan S. Ryerse*
Affiliation:
St. Louis University Health Sciences Center
Paul C. C. Feng
Affiliation:
Monsanto Company
R. Douglas Sammons
Affiliation:
Monsanto Company
*
[email protected]

Extract

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Various fluorescent stains and vital dyes have been used to identify dead cells in animal tissues and celi lines. In plants, fluorescein diacetate and propidium iodide have been used to label nuclei and to identify necrotic cells in plant protoplasts and 4,6-diamidino-2-phenylindole (DAPI) has been used to mark senescing cells in sections of roots. However, these dyes may be problematic when used with intact plant tissue with well-developed cells walls which may impede dye penetration. Endogenous fluorescence has been used to identify dead cells in intact and sectioned plant tissues. Published procedures typically employ ultraviolet (UV) excitation wavelengths of 340-380 nm and emission wavelengths of 400- 425 nm, thus requiring a UV filter set.

Type
Research Article
Information
Microscopy Today , Volume 9 , Issue 2 , March 2001 , pp. 22 - 24
Copyright
Copyright © Microscopy Society of America 2001

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