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Endogenous Fluorescence Identifies Dead Cells In Plants

Published online by Cambridge University Press:  14 March 2018

Jan S. Ryerse ,
Paul C. C. Feng  and
R. Douglas Sammons
Jan S. Ryerse*
Affiliation:
St. Louis University Health Sciences Center
Paul C. C. Feng
Affiliation:
Monsanto Company
R. Douglas Sammons
Affiliation:
Monsanto Company
*
[email protected]

Extract

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Various fluorescent stains and vital dyes have been used to identify dead cells in animal tissues and celi lines. In plants, fluorescein diacetate and propidium iodide have been used to label nuclei and to identify necrotic cells in plant protoplasts and 4,6-diamidino-2-phenylindole (DAPI) has been used to mark senescing cells in sections of roots. However, these dyes may be problematic when used with intact plant tissue with well-developed cells walls which may impede dye penetration. Endogenous fluorescence has been used to identify dead cells in intact and sectioned plant tissues. Published procedures typically employ ultraviolet (UV) excitation wavelengths of 340-380 nm and emission wavelengths of 400- 425 nm, thus requiring a UV filter set.

Type
Research Article
Information
Microscopy Today , Volume 9 , Issue 2 , March 2001 , pp. 22 - 24
Copyright
Copyright © Microscopy Society of America 2001

References

1. Haugland, R. P. 1992. Handbook of fluorescent probes and research chemicals. Molecular Probes, Inc., Eugene, Oregon, USA.

2. Sheehan, D. C. and Hrapchak, B. B.. 1980. Theory and Practice of Histotechnology. 2nd edition, Batelle Press, Columbus, Ohio, p 123.Google Scholar

3. Rayburn, A. L, Auger, J. A. and McMurphy, L. M., 1992. Estimating percentage constitutive heterochromatin by flow cytometry. Exp. Cell Res, 198:175-178.Google Scholar

4. Widholm, J. M, 1972. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech. 47:189-194.Google Scholar

5. Yamada, S., Katsuhara, M., Kelly, W. B., Michalowski, C. B. and Bohnert, H. J.. 1995. A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant The Plant Celt 7:1129-1142.Google Scholar

6. O'Brien, T. P. and McCully, M. E.. 1981. The Study of Plant Structure: Principles and Selected Methods. Termarcarphi Printing Ltd., Melbourne, Australia.Google Scholar

7. Bowling, S. A., Clarke, J. D., Liu, Y., Klessig, D. F. and Dong, X.. 1997. The cpr5 mutant of Arabadopsis expresses both NPR1-dependent and NPR1-independent resistance. The Plant Cell 9:1573-1584.Google Scholar

8. Kosslak, R. M., Chamberlin, M. A., Palmer, R. G. and Bowen, B. A.. 1997, Programmed cell death in the root cortex of soybean root necrosis mutants. The Plant Journal 11:729-745.Google Scholar

9. Shah, J. P. Kachroo, and Klessig, D. F., 1999. The Arabadopsis ssil mutation restores pathogenesis-related gene expression in nprl plants and renders defensin gene expression salicylic acid dependent. The Plant Cell 11:191-206.Google Scholar

10. Raven, P. H., Evert, R. F. and Eichhorn, S. E.. 1999. Biology of Plants. Sixth edition, W. H. Freeman and Co., New York, New York, p 131.Google Scholar