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Detecting Bacteria in Wood using a Fluorescent Lipid Probe
Published online by Cambridge University Press: 14 March 2018
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Wood cells have strong autofluorescence in a wide wavelength band due to lignin in the cell walls. The detection of microorganisms in wood is very difficult when using fluorescent microscopy because of interferences. We have recently developed fluorescent staining techniques to differentiate fungal hyphae from wood cell walls (Singh, et al., 1997; Xiao, et al., 1997). This study was aimed at developing fluorescent techniques to visualize bacteria in wood using confocal laser scanning microscopy (CLSM). Nitrobenzoxadiazole glycerophosphoethanolamine (NBD-PE, Molecular Probes), a widely used membrane probe which accords strong fluorescence upon lipids, was compared with glutaraldehyde which had proved useful in our initial attempts to visualize fungal hyphae in wood because of the cell auto fluorescence it causes (Singh et al., 1997).
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- Research Article
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- Copyright © Microscopy Society of America 1998
References
Haugland, R. P., 1996. Handbook of Fluorescent Probes and Research Chemicals (Sixth Edition). Molecular Probes, Inc., Eugene.Google Scholar
Singh, A. P. and Butcher, J. A., 1991, Bacterial degradation of wood celi walls: A review of degradation patterns, J. Inst. Wood Sci, 12(3), 143–157.Google Scholar
Singh, A. P., Xiao, Y. and Wakeling, R.N.. 1997. Glutaraldehyde autofluorescence useful in confocal studies of fungi. Microscopy in Focus Newsletter for Microscopy New Zealand Inc. No.2).CrossRefGoogle Scholar
Xiao, S.Y., Singh, A.P. and Wakeling, R.N.. 1997; Detection of fungal hyphae in wood using a chitin selective fluorescent probe. Microscopy in Focus Newsletter for Microscopy New Zealand Inc. No,3).Google Scholar
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