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A cryoSEM Method for Preservation and Visualization of Calcified Shark Cartilage (And Other Stubborn Heterogeneous Skeletal Tissues)

Published online by Cambridge University Press:  14 March 2018

Mason N. Dean ,
Stanislav N. Gorb  and
Adam P. Summers
Mason N. Dean*
Affiliation:
Ecology & Evolutionary Biology, University of California Irvine, Irvine, CA USA
Stanislav N. Gorb
Affiliation:
Evolutionary Biomaterials Group, Max Planck Institute for Metals Research, Stuttgart, Germany
Adam P. Summers
Affiliation:
Ecology & Evolutionary Biology, University of California Irvine, Irvine, CA USA
*
[email protected]

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Biological materials interest biologists and engineers for their complex interactions among constituents and unique mechanical properties. While the heterogeneous tissue structure and its material properties are responsible for intriguing biomechanics, they pose challenges for sectioning, particularly in regions with stark tissue boundaries. Microscopists level this playing field when sectioning samples by embedding them in paraffin or plastic; but for scanning electron microscopy, natural morphology must be preserved without sacrificing the sample’s surface contours. Here we outline a simple preparation method for visualizing in cryoSEM the calcified cartilage of sharks and rays (elasmobranch fishes), a layered biocomposite that has traditionally been considered difficult to prepare for microscopy.

Type
Research Article
Information
Microscopy Today , Volume 16 , Issue 6 , November 2008 , pp. 48 - 51
Copyright
Copyright © Microscopy Society of America 2008

References

Applegate, S. P. (1967). A survey of shark hard parts. In Sharks, Skates and Rays, eds. Gilbert, P. W. Mathewson, R. F. and Rall, D. P., pp. 37-66. Maryland: Johns Hopkins Press.Google Scholar
Clement, J. G. (1992). Re-examination of the fine structure of endoskeletal mineralization in Chondricthyes: implications for growth, ageing and calcium homeostasis. Australian Journal of Marine and Freshwater Research 43, 157-181.Google Scholar
Dean, M.N. and Summers, A.P., (2007). Ontogeny, morphology and mechanics of the tessellated skeleton of cartilaginous fishes. Integrative and Comparative Biology. 47: e27.Google Scholar
Dean, M.N. (2007) Ontogeny, morphology and mechanics of the tessellated skeleton of cartilaginous fishes. Journal of Morphology. 268 (1066).Google Scholar
Dean, M. N., Chiou, W.-A. and Summers, A. P. (2005). Morphology and ultrastructure of prismatic cartilage calcification. Microscopy and Microanalysis 11, 1196-1197.CrossRefGoogle Scholar
Dean, M. N. and Summers, A. P. (2006). Cartilage in the skeleton of cartilaginous fishes. Zoology 109, 164-168.Google Scholar
Dingerkus, G., Seret, B. and Guilbert, E. (1991). Multiple prismatic calcium phosphate layers in the jaws of present-day sharks (Chondrichthyes; Selachii). Experientia 47, 38-40.Google ScholarPubMed
Eames, B.F., Allen, N., Young, J., Kaplan, A., Helms, J.A. & Schneider, R.A. (2007) Skeletogenesis in the swell shark Cephaloscyllium ventriosum. Journal of Anatomy 210: 542-554.CrossRefGoogle Scholar
Gorb, S.N. (2006). Fly microdroplets viewed big: a Cryo-SEM approach. Microscopy Today 14(5): 38-39 Google Scholar
Hunziker, E. B., Herrmann, W., Schenk, R. K., Mueller, M. and Moor, H. (1984). Cartilage ultrastructure after high-pressure freezing, freeze substitution, and low-temperature embedding .1. Chondrocyte ultrastructure - implications for the theories of mineralization and vascular invasion. Journal of Cell Biology 98, 267-276.CrossRefGoogle Scholar
Hunziker, E. B., Michel, M. and Studer, D. (1997). Ultrastructure of adult human articular cartilage matrix after cryotechnical processing. Micros. Research and Technique 37, 271-284.3.0.CO;2-O>CrossRefGoogle Scholar
Kemp, N. E. and Westrin, S. K. (1979). Ultrastructure of calcified cartilage in the endoskeletal tesserae of sharks. Journal of Morphology 160, 75-102.CrossRefGoogle Scholar
Studer, D., Michel, M., Wohlwend, M., Hunziker, E.B. and Buschmann, M.D. (1995). Vitrification of articular cartilage by high-pressure freezing. Journal of Microscopy. 179(3):321-32.CrossRefGoogle Scholar
Summers, A. P. (2000). Stiffening the stingray skeleton - An investigation of durophagy in myliobatid stingrays (Chondrichthyes, Batoidea, Myliobatidae). Journal of Morphology 243, 113-126.3.0.CO;2-A>CrossRefGoogle Scholar
Tavakol, K., Miller, R.G., Bazett-Jones, D.P., Hwang, W.S., McGann, L.E. and Schachar, N.S. (1993) Ultrastructural changes of articular cartilage chondrocytes associated with freeze- thawing. Journal of Orthopedic Research. 11(1): 1-9.CrossRefGoogle Scholar