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Creating Pseudocolor Images using ImageJ

Published online by Cambridge University Press:  14 March 2018

Joel B. Sheffield*
Affiliation:
Department of Biology, Temple University, Philadelphia PA

Extract

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It is common for microscopists to examine fluorescent samples that have been exposed to a mixed set of fluorophores with specific staining properties. Images are often captured from these samples as a set, using different filter sets to isolate specific elements of the sample. Sometimes the images are captured in gray scale, and in other cases, they are captured with color cameras. In either case, one would often like to combine the images to create a single image that presents the information from all fluorophores at once. This kind of display is particularly useful for determining the relative locations of different components.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2006

Footnotes

Editor's Note: The author and Tony Collins, McMaster Univ, will be chairing a session on ImageJ at M&M-2007 in Fort Lauderdale.