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Colocalization of Fluorescent Probes Using Image-Pro® Plus v. 5.0

Published online by Cambridge University Press:  14 March 2018

Will Casavan  and
Yuri Gaidoukevitch
Will Casavan*
Affiliation:
Media Cybernetics, Inc.
Yuri Gaidoukevitch*
Affiliation:
Media Cybernetics, Inc.
*
[email protected]
[email protected]

Extract

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Fluorescence labeling techniques are quite useful to microscopists. Not only can fluorescent probes label sub-cellular structures with a high degree of specificity, they can also do so with a very high degree of sensitivity. This combination of specificity and sensitivity permits observations of cellular events that are not always visible using traditional methodologies, such as brightfield microscopy.

Type
Research Article
Information
Microscopy Today , Volume 11 , Issue 6 , December 2003 , pp. 48 - 50
Copyright
Copyright © Microscopy Society of America 2003

References

Manders, E.M.M., Verbeek, F.J.,Aten, J.A., Measurement of colocalization of objects in dualcolor confocal images. Journal of Microscopy 169:375-3S2 (1993).CrossRefGoogle Scholar
Smallcombe, A., McMillan, D., Bio-Rad Technical Note 11. Colocalization; how it is determined, and how it is analysed with ths Blo-Rad LaserPix image analysis software?Google Scholar