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Cellulose Acetate Replication of Plant Surfaces for SEM

Published online by Cambridge University Press:  14 March 2018

Gib Ahlstrand*
Affiliation:
University of Minnesota

Extract

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Replicas of botanical surfaces are sometimes preferred for imaging in the SEM, instead of original surfaces, in cases where it is not possible or desirable, for a variety of reasons, to prepare originais using conventional techniques (chemical fixation followed by dehydration and critical point drying, freeze drying, or cryo-SEM). Samples may be rare (herbarium specimens), or impossible to bring into the lab for processing, and field replication may be the only alternative.

Type
Microscopy 101
Copyright
Copyright © Microscopy Society of America 2003

References

1. Kay, Desmond H.,ed. 1965. Tech, for Electron Microscopy, pp. 96-152.Google Scholar
2. North, C. 1956. A technique for measuring structural features of plant epidermis using cellulose-acetate films. Nature 178:1186-1187.Google Scholar