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Big is Beautiful: Science (and Art) in Wood Microscopy

Published online by Cambridge University Press:  14 March 2018

Nigel Chaffey
Nigel Chaffey*
Affiliation:
Swedish University of Agricultural Sciences, Umeå, Sweden

Abstract

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Notwithstanding the importance of trees, considerable technical problems have severely restricted study of the fine structure of the vascular cambium and the wood and bark that it produces. And despite the advent of immunocytochemical techniques, it is only in the last few years that the roie of the cytoskeleton in the process of secondary vascular differentiation has been studied. Yet, for those who persist with this intractable material, the experience can be rewarding. This article discusses the difficulties involved in microscopy of this material, and offers some solutions.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 9 , November 1997 , pp. 12 - 15
Copyright
Copyright © Microscopy Society of America 1997

References

Barnett, J.R., 1992, Reactivation of the cambium in Aesculus hippocastanum L: a transmission electron microscope study. Ann, Bot. 70; 169–177,Google Scholar
Carnegie, J.A., McCully, M.E., Robertson, HA, 1980. Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a iabile tissue protein. J. Hist. Cyto. 28; 308–310.Google Scholar
Chaffey, M.J, Barlow, P.W., Barnett, J.R., 1996, Microtubularcytoskeleton of vascular cambium and its derivatives in roots of Aesculus bippocastanum L (Hippocastanaceae), In: LA, Donaldson, B.G. Butterfield, P.A. Singh, LJ, Whitehouse, eds. Recent advances in wood anatomy, New Zealand Forest Research Institute, Rotorua, pp.171–183.Google Scholar
Chaffey, N.J., Barnett, J.R., Barlow, P.W., 1997a. Cortical microtubule involvement in bordered pit formation in secondary xylem vessel elements of Aescufus hippocastanum L (Hippocastanaceae): a correlative study using electron microscopy and indirect immunofluorescence microscopy. Protoplasma 197; 64–75.Google Scholar
Chaffey, N.J., Bamett, J.R, Barlow, P.W., 1997b. Endomembranes, cytoskeleton, and cell walls: aspects of the ultrastructure of the vascular cambium of taproots of Aesculus hippocastanum L, (Hippocastanaceae). Int. J. Plant Sci. 158; 97–109.Google Scholar
Chaffey, N.J., Barnett, J.R., Barlow, P,W., 1997c, Visualization of the cytoskeleton within the secondary vascular system of hardwood species. J, Microsc, 187; 77–84.Google ScholarPubMed
Crowley, H.H., 1997, Pretreating epoxy thin sections with sodium periodate prior to immunostaining, Micro, Today 97-6; 24–25.Google Scholar
Farrar, J.J., Evert, R.F., 1997. Seasonal changes in the ultrastructure of the vascular cambium of Robinia pseudoacacia. Trees 11; 191–200.Google Scholar
Funada, R., Abe, H, Furusawa, O, Imaizumi, H., Fukuzawa, K, Ohtani, J., 1997, The orientation and localization of cortical microtubules in differentiating conifer tracheids during cell expansion. Plant Cell Physiol. 38; 210–212.Google Scholar
O'Brien, T.P., McCully, M. E., 1981, The study of plant structure: principles and selected methods. Termacarphi, Melbourne.Google Scholar
Scanalytics, Inc., 1997. High resolution 3-D fluorescence microscopy: a comparison of confocal laser scanning microscopy and a wide-field deconvolution technique. Micro, Today 97-6; 10-12,CrossRefGoogle Scholar
Sharon, E., Spiegel, Y., 1996. Gold-conjugated reagents for the labelling of carbohydrate-recognition domains and giycoconjugates on nematode surfaces. J. Nematol. 28; 124–127.Google Scholar