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Avoiding Etching of Silver When Using Silver Staining With OsO4 Fixation

Published online by Cambridge University Press:  14 March 2018

Rick Powell
Rick Powell*
Affiliation:
Nanoprobes, Inc.

Extract

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Post-fixing with osmium tetroxide before silver-staining sections causes problems because the OsCU can etch the deposited silver. However, it is not a good idea to perform silver enhancement after osmium tetroxide treatment because the deposited osmium can also act as a nucleating site for silver deposition, and the osmicated regions of the sample will be silver enhanced. If gold-toning is used after OsO4 post-fixation, the OSO4 can also nucleate gold particles. OsO4 should always come after silver enhancement.

Type
Research Article
Information
Microscopy Today , Volume 7 , Issue 7 , September 1999 , pp. 22 - 23
Copyright
Copyright © Microscopy Society of America 1999

References

1) Burry, R.W. Pre-embedding immunocytocrtemistry with silverenhanced small gold particles. Chap. 14, pp. 217–230. In Hayat, M. A. ed. 1995. Immunogold silver staining: Principles, methods and applications. CRC Press, Boca Raton.Google Scholar
2) Arai, R., et at. Brain Res. Bull., 1992, 28, 343–345.Google Scholar
3) Arai, R., and Nagatsu, I. 1995. in: Immunogold-Silver Staining: principles, Methods and Applications. Hayat, M. A., ed. Chap. 13, pp 209–216. CRC Press, Boca Raton. FL).Google Scholar
4) Sawada, H., and Esaki, J. 1994. Elect. Micro., 43, 361–66.Google Scholar