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Automated, Robotic Preparation of Vitrified Samples for 2D and 3D Cryo Electron Microscopy

Published online by Cambridge University Press:  14 March 2018

Peter. M. Frederik  and
Marc M.H. Storms
Peter. M. Frederik*
Affiliation:
University Maastricht, EM-unit, Universiteitssingel 50, 6229 ER Maastricht, The Netherlands
Marc M.H. Storms*
Affiliation:
FEI Company, Achtseweg Noord 5, 5600 KA Eindhoven, The Netherlands
*
[email protected]
[email protected]

Extract

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Fundamental research within the scope of cell and structural biology as well as nano-technology is increasingly focusing on unraveling interactive biological and biochemical processes and pathways at the macromolecular level. For this, high resolution transmission electron microscopy (TEM) is indispensable. Of paramount importance is the three-dimensional visualization of macromolecular structures and molecular machines in their native hydrated state. Their physical fixation within ultra-thin vitrified ice layers is the crucial starting point.

Type
Research Article
Information
Microscopy Today , Volume 13 , Issue 6 , November 2005 , pp. 32 - 39
Copyright
Copyright © Microscopy Society of America 2005

References

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