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3-D Reconstruction of Thick IVEM Samples Using Tuned Aperture Computed Tomography® (TACT®)

Published online by Cambridge University Press:  14 March 2018

W. Gray Jerome ,
Ken Grant ,
Patricia G. Yancey ,
A. M. Al Gailany ,
Wolfram Betterman  and
Richard L. Webber
W. Gray Jerome
Affiliation:
Wake Forest University School of Medicine
Ken Grant
Affiliation:
Wake Forest University School of Medicine
Patricia G. Yancey
Affiliation:
Wake Forest University School of Medicine
A. M. Al Gailany
Affiliation:
Wake Forest University School of Medicine
Wolfram Betterman
Affiliation:
Wake Forest University School of Medicine
Richard L. Webber
Affiliation:
Wake Forest University School of Medicine

Extract

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Most objects in our world are 3-dimensionai (3-D), and this is certainly true of cellular ultrastructure. The challenge in microscopy has always been how to analyze this 3-D information. Traditional thin-section microscopy has a minimal ability to view 3-D structure, because it is limited to viewing thin, almost twodimensional (2-D) planes taken from the specimen. The development of high and intermediate voltage electron microscopes (HVEM and IVEM) provides the ability to investigate the ultrastructure of thick biological samples—allowing a unique view of the 3-D interrelationships of cells and organelles.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 4 , May 2000 , pp. 26 - 29
Copyright
Copyright © Microscopy Society of America 2000

References

1. Perkins, G. A., Renken, C., Song, J., Frey, T., Young, S., Lamont, S., Martone, M., Lindsey, S., and Ellisman, M.. 1997. Electron tomography of large, multicomponent biological structures. J Struct Biol. 120:219-27.CrossRefGoogle ScholarPubMed

2. Koster, A., Grimm, R., Typke, D., Hegerl, R., Stoschek, A., Walz, J., and Baumeister, W.. 1997. Perspectives of molecular and cellular electron tomography. J Struct Biol. 120:276-308.CrossRefGoogle ScholarPubMed

3. Frank, J. 1996, Three-dimensional electron microscopy of macromolecular assemblies, Academic Press, San Diego.Google Scholar

4. Grant, D. G. 1972. Tomosynthesis: a three-dimensional radiographic imaging technique. IEEE Trans Biomed Engineering. 19:20-28.Google ScholarPubMed

5. Webber, R., Horton, R., Tyndall, D., and Ludlow, J.. 1997. Tunedaperture computed tomography (TACT). Theory and application for three-dimensional dentoatveolar imaging. Dentomaxillifaciai Radiol. 26:53-62.Google ScholarPubMed

6. Webber, R., Underhill, H., and Freimanis, R.. 2000. A controlled evaluation of tuned-aperture computed tomography applied to digital spot mammography. J Digit Imaging. 13:1-9.CrossRefGoogle ScholarPubMed

7. Jerome, W. G., and Lewis, J. C.. 1985. Early atherogenesis in White Carneau pigeons II Ultrastructural and cytochemical observations. Am J Pathoi. 119:210-222.Google ScholarPubMed

8. Gomori, G. 1950. An improved histochemical technic for acid phosphatase. Stain Technol. 25:81-85.Google Scholar

* TACT is copyrighted by Wake Forest University School of Medicine. TACT requires a 486 or better PC running Windows (95, NT 4.0, or higher) and sufficient RAM to handle large image files. The program is still in the developmental stages. Anyone interested in working with the program for research purposes or in commercial applications is encouraged to contact Dr. Jay Jerome ([email protected]) or Wake Forest University's Technology Asset Management Office (in care of Dr. William H. Williams, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157.